| 产品介绍 | | |
| Mouse anti glucose transporter 4 antibody, clone 1F8originally raised against rat intracellular low density microsomes (Jameset al.1987) recognizes an epitope in the cytoplasmic region of Glucose transporter 4 (GLUT4), an insulin-regulated facilitative glucose transporter found in adipose tissue and striated muscle. When stimulated by insulin, GLUT4 translocates from intracellular stores to the cell surface, facilitating passive diffusion of circulating glucose into muscle and fat cells. GLUT4 is also stimulated to locate to the cell surface by muscle contraction, particularly in cardiac muscle (Jameset al.1988).Mouse anti glucose transporter 4 antibody, clone 1F8 has been used successfully to demonstrate the localization of GLUT4 to the basolateral side of ductal structures in the rat submandibular salivary gland in formalin fixed, paraffin embedded material (Cetiket al.2014). |
| 产品详情 | | |
Target Species | Rat |
Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin |
Approx. Protein Concentrations | IgG concentration 0.1mg/ml |
Immunogen | Partially purified vesicles containing insulin-responsive glucose transporter 4. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Max Ex/Em | 490/525 |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Immunofluorescence | √ | Neat | 1/25 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
| 数据 |
| HeLa cells stained with Mouse anti Glucose Transporter 4:FITC (4670-1725F) and counterstained with DAPI. |
| 文献 |
| 1. James, D.E.et al.(1988) Insulin-regulatable tissues express a unique insulin-sensitive glucose transport protein.Nature. 333 (6169): 183-5.2. James, D.E.et al.(1989) Molecular cloning and characterization of an insulin-regulatable glucose transporter.Nature. 338 (6210): 83-7.3. Cleasby, M.E.et al.(2003) Programming of rat muscle and fat metabolism byin uterooverexposure to glucocorticoids.Endocrinology. 144 (3): 999-1007.4. Huang, J.,et al. (2001) Insulin can regulate GLUT4 internalization by signaling to Rab5 and the motor protein dynein.Proc Natl Acad Sci U S A. 98:13084-13089.5. Farese, R.V.et al.(2007) Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes.J Clin Invest. 117: 2289-301.6. Grainger, D.L.et al.(2011) Involvement of phosphatidylinositol 5-phosphate in insulin-stimulated glucose uptake in the L6 myotube model of skeletal muscle.Pflugers Arch. 462: 723-32. |
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