| 产品介绍 | | |
| Rabbit anti FITC antibodyrecognizes fluorescein and does not react with immunoglobulins from any species.Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. |
| 产品详情 | | |
Target Species | Chemical |
Product Form | Purified Ig - liquid |
Preparation | Purified IgG prepared by immunoaffinity chromatography |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.1%Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Immunogen | FITC Isomer 1. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
Immunohistology - Frozen | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
| 二抗相关产品 |
Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
Goat anti Rabbit IgG (H/L):HRP | STAR124P | C;E;WB | 1 mg |
Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| 数据 |
| Published customer image:Rabbit anti FITC antibody (4510-7804) used as a visualization antibody in an ELISA assay for the detection of antiFasciola hepaticaantibodies in human sera.Image caption:Comparison of the detection limit of the MM3-SERO and SeroFluke tests. The ability of MM3-SERO ELISA (A) and SeroFluke (B) to detect anti-Fasciola antibodies in two fold dilutions of pooled positive sera was compared. The starting serum dilution was 1/100 for both tests. |
| 文献 |
| 1. van der Loos, C. (2008) Multiple immunoenzyme staining: methods and visualizations for the observation with spectral imaging.J. Histochem Cytochem. 56: 313-328.2. Bdeir, K.et al.(2010) Neutrophil alpha-defensins cause lung injury by disrupting the capillary-epithelial barrier.Am J Respir Crit Care Med. 181: 935-46.3. Hsiao, J.R.et al.(2010) Cyclic alphavbeta6-targeting peptide selected from biopanning with clinical potential for head and neck squamous cell carcinoma.Head Neck. 32 (2): 160-72.4. Türeci Oet al.(2011) Claudin-18 gene structure, regulation, and expression is evolutionary conserved in mammals.Gene. 481 (2): 83-92.5. Martínez-Sernández, V.et al.(2011) Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis.PLoS Negl Trop Dis. 5 (11): e1376. |
QQ:1749072012