| 产品介绍 | | |
| Sheep anti Human fibrinogen antibodyrecognizes human fibrinogen, a complex ~340 kDa hetero-hexameric (di-trimeric) glycoprotein consisting of 3 pairs of α, β and γ chains linked by a series of 29 disulphide bonds (Henschenet al.1983). The six chains are arranged in such a way that all the N-Terminal ends adjoin to form a centralE domainwith two trimeric coiled coil structures connecting to outer D domains. Fibrinogen plays an important role in the coagulation process with the D and E domains interacting via the C-Terminal ends of the α chains during fibrin clot cross-linking.Sheep anti human fibrinogen antibody shows minimal cross-reactivity with related serum proteins. Fibrinogen has been identified as a ferritin binding protein in the horse (Orinoet al.1993). Sheep anti human fibrinogen antibody has been successfully as a capture reagent for ferritin - anti ferritin IgG complexes in horse plasma to evaluate the antibody response to ferritin by ELISA (Takahashiet al.2013). |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by ion exchange chromatography |
Buffer Solution | Glycine buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3)<0.1% EACA<0.01% Benzamidine<1mM EDTA |
Approx. Protein Concentrations | 5.0 mg/ml |
Immunogen | Human fibrinogen purified from plasma. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/5000 | 1/50000 |
Immunofluorescence | √ | 1/200 | 1/400 |
Immunohistology - Frozen | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C;E;F;WB | 1.5 ml |
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| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C;E;P;WB | 1 ml |
| 数据 |
| Published customer image:Sheep anti Human fibrinogen antibody (4440-8004) used for the evaluation of fibrinogen deposition in mouse brain by immunofluorescence.Image caption:Inhibition of 2-AG hydrolysis reduces LPS-induced BBB permeability. a, b Fibrinogen levels in b plasma and the a ratio of brain to plasma fibrinogen were assessed by ELISA. n = 5/7 mice per group. c, d Fluorescent immunostaining in the striatum for fibrinogen (red) and vascular marker (CD31; green) demonstrated leakage of fibrinogen into the brain with vehicle treatment, whereas vascular integrity was preserved when (e, f) MAGL was inhibited. g Extravascular fibrinogen was semi-quantitated in fluorescently labeled sections of the striatum. Bar graphs were plotted with mean ±SEM and data analyzed using one-way analysis of variance (ANOVA) with Tukey post-hoc comparisons. n = 5/7 mice per group. Significance is shown as *p <0.05, **p <0.01. Scale bar = 20 μm. |
| 文献 |
| 1. Brill, A.et al.(2011) von Willebrand factor-mediated platelet adhesion is critical for deep vein thrombosis in mouse models.Blood. 117: 1400-7.2. Barrera, V.et al.(2011) Host fibrinogen stably bound to hemozoin rapidly activates monocytes via TLR-4 and CD11b/CD18-integrin: a new paradigm of hemozoin action.Blood. 117: 5674-82.3. Grainger, D.J.et al.(2004) Apolipoprotein E modulates clearance of apoptotic bodies in vitro and in vivo, resulting in a systemic proinflammatory state in apolipoprotein E-deficient mice.J Immunol. 173: 6366-75.4. Grainger, D.J.et al.(2001) Suppressing Thrombin Generation is Compatible With the Development of Atherosclerosis in MiceThromb Res. 102: 71-80.5. Chien, H.W. (2013) Surface conjugation of zwitterionic polymers to inhibit cell adhesion and protein adsorption.Colloids Surf B Biointerfaces. 107: 152-9.6. Plskova, J.et al.(2004) Quantitative evaluation of the corneal endothelium in the mouse after grafting.Br J Ophthalmol. 88: 1209-16. |
QQ:1749072012