| 产品介绍 | | |
| Sheep anti Human C3c antibodydetects human complement 3c, a polypeptide fragment which is present in C3. C3 plays a central role in the activation of the complement system. Processing of C3 by C3 convertase is the central reaction in both classical and alternative complement pathways, resulting in C3a and C3b. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates, and help to initiate the complement cascade, potentially resulting in cellular apoptosis. C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c and C3dg. Defects in C3 can result in susceptibility to pyogenic infection.This antbody shows minimal reactivity with related serum proteins. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 5.0 mg/ml |
Immunogen | Native human C3c purified from serum. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/400 | |
Immunohistology - Frozen | √ | 1/50 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls. |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C;E;F;WB | 1.5 ml |
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F;IF | 0.1 mg |
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C;E;P;WB | 1 ml |
| 数据 |
Native Human C3c detected using Sheep anti Human C3c:FITC (2222-6604F). |
| 文献 |
| 1. Janssen, B.J.et al.(2005) Structures of complement component C3 provide insights into the function and evolution of immunity.Nature. 437 (7058): 505-11.2. Lewis, L.A.et al.(2019) Effect of a C1s Inhibitor on the Efficacy of Anti-Capsular Antibodies againstNeisseria meningitidisandStreptococcus pneumoniae.Immunohorizons. 3 (11): 519-30. |
QQ:1749072012