| 产品介绍 | | |
| Collagen type I 90%Collagen type III 10%Non-collagenous proteins <0.5% |
| 产品详情 | | |
Target Species | Bovine |
Product Form | Purified protein from calf tendon - lyophilized |
Preparation | Bovine collagen type I was isolated from calf tendons. Isolation steps included extraction into dilute acetic acidafter pepsin treatment, followed by multiple steps of differential salt precipitation and ion-exchange chromatography. |
Buffer Solution | Essentially salt free |
Preservative Stabilisers | None present |
Protein Molecular Weight | ~300 kDa |
Reconstitution | Use 0.5M acetic acid, pH 3.0. Dispersed collagen have to be thoroughly homogenized in a Potter after addition of the solute and left overnight in the coldroom to dissolve.Dissolved collagen retains immunologic properties of native collagen type I. Thermal denaturation (5 minutes at 100ºC) converts dissolved collagen to gelatin. |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
| 存储条件 | | |
| Prior to reconstitution store at +4℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted.Avoid repeated freezing and thawing as this may denature the antibody.Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
QQ:1749072012