Mouse anti Human ZAP 70:FITC
货号：1065002S
规格：25 µg
价格：2227
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：ZAP 70
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：SBZAP
荧光染料：FITC
产品介绍 | | |
| Mouse anti Human ZAP 70 antibody, clone SBZAPrecognizes human ZAP-70, a 70kDa tyrosine protein kinase which associates with the T-cell receptor (TCR) zeta chain and undergoes phosphorylation following TCR stimulation. ZAP-70 is primarily expressed in T lymphocytes and natural killer cells, where it plays a key role in T-cell receptor (TCR) signalling. Recent studies suggest that ZAP-70 is also expressed in a population of normal immature B-cells and in B-cells from a sub-set of patients with chronic lymphocytic leukaemia. |
产品详情 | | |
Target Species | Human |
Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Immunogen | Synthetic peptide corresponding to amino acids 280-309 of human ZAP70 conjugated to keyhole limpet hemocyanin (KLH). |
Approx. Protein Concentrations | IgG concentration 0.1 mg/ml |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
Regulatory | For research purposes only |
Guarantee | Guaranteed until date of expiry. Please see product label. |
存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.- 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™(Product CodeBUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
相关产品 |
Description | Product Code | Applications | Pack Size |
Human Seroblock | BUF070A | F | 50 Test |
Human Seroblock | BUF070B | F | 200 Test |
Leucoperm | BUF09 | F | 50 Tests |
Leucoperm | BUF09B | F | 250 Tests |
Leucoperm | BUF09C | F | 1000 Tests |
文献 |
| 1.Roifman, C.M.et al.(1989) Depletion of CD8+ cells in human thymic medulla results in selective immune deficiency.J Exp Med. 170 (6): 2177-82.2.Arpaia, E.et al.(1994) Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase.Cell. 76 (5): 947-58. |
技术参数 产品应用 F*
产品类型 Monoclonal Antibody
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