| 产品介绍 | | |
| Rabbit anti Human ACTH antibodyrecognizes human adrenocorticotrophic hormone, also known as corticotrophin. ACTH is a 4.5 kDa hormone derived from cleavage of pro-opiomelanocortin (POMC), produced in the pituitary gland in response to corticotrophin releasing hormone (CRH) activity and functions to stimulate release of adrenal cortisol.Rabbit anti Human ACTH polyclonal antibody is directed against the N-terminal portion of adrenocorticotrophic hormone and also recognizes the ACTH cleavage fragment α-melanocyte stimulating hormone (α-MSH), responsible for melanin pigmentation control in most vertebrate species (Fiordelisioet al.2006). Rabbit anti Human ACTH does not recognize the C-terminal ACTH cleavage product, corticotropin-like intermediary peptide (CLIP). |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified Ig - liquid |
Preparation | Purified Ig prepared by affinity chromatography on Protein G |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09%Sodium Azide (NaN3) |
Approx. Protein Concentrations | 5.0 mg/ml |
Immunogen | Synthetic peptide corresponding to the amino acids 1-24 (SYSMEHFRWGKPVGKKRRPVKVYP) of ACTH conjugated to Keyhole limpet Haemocyanin. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/500 | 1/1000 |
Immunofluorescence | √ | | |
| Immunohistology - Paraffin | √ | 1/100 | 1/200 |
Immunohistology - Frozen | √ | 1/100 | 1/200 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.Histology Positive Control TissueACTH-containing cells of the anterior pituitary gland. |
| 二抗相关产品 |
Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E;WB | 1 mg |
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | Flow | 1 mg |
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | E;WB | 1 mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C;E;WB | 1 mg |
| Sheep anti Rabbit IgG:FITC | STAR34B | C;F | 1 mg |
| Sheep anti Rabbit IgG:RPE | STAR35A | Flow | 1 ml |
| 文献 |
| 1. Osamura, R.Y.et al.(1980) Adrenocorticotropic hormone cells and immunoreactive beta-endorphin cells in the human pituitary gland: normal and pathologic conditions studied by the peroxidase-labeled antibody method.Am J Pathol. 99 (1): 105-24.2. Fiordelisio, T.et al.(2006) Cells of proopiomelanocortin lineage from the rodent anterior pituitary lack sexually dimorphic expression of neurofilaments.Neuroendocrinology. 83:360-70.3. Farris, G.M.et al. (2007) Recombinant rat and mouse growth hormones: risk assessment of carcinogenic potential in 2-year bioassays in rats and mice.Toxicol Sci. 97: 548-61. |
QQ:1749072012