Goat anti Human Collagen I:Biotin
货号：131008
规格：0.2 mg
价格：7497
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Collagen I
物种：人
宿主：山羊
抗体亚型：IgG
荧光染料：Biotin
| 产品介绍 | | |
| Goat anti Human collagen I antibodyrecognizes human type 1 collagen.Goat anti Human collagen I antibody has been cross-adsorbed against collagen types II, III, IV, V and VI. Results from ELISA demonstrate <10% cross-reactivity with other collagen types. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG conjugated to Biotin - liquid |
Antiserum Preparation | Antisera to human type 1 collagen were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Approx. Protein Concentrations | Ig concentration 0.4 mg/ml |
Immunogen | Human type 1 collagen. |
Regulatory | Human type 1 collagen. |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/200 | 1/2000 |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 数据 |
| Sandwich ELISA analysis of human ferritin using Mouse anti Human collagen I/II/III antibody, clone NLI/22 (2150-2203) as a capture reagent and biotinylated Mouse anti Human collagen I antibody, (131008) as a detection reagent with native human collagen (2150-0030) as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the Bio-Rad iMark Microplate Absorbance Reader (Bio-Rad Catalog 168-1135). Normal serum (green) and plasma (orange) samples are included diluted 1:4. |
技术参数 产品应用 ELISA
产品类型 Polyclonal Antibody
推荐用于 ELISA
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