Mouse IgG2a Negative Control:FITC
货号：MCA1210F
规格：0.1 mg
价格：2695
产品类型：小鼠同型对照
品牌：Bio-Rad
物种：其它
宿主：小鼠
抗体亚型：IgG2a
荧光染料：FITC
| 产品介绍 | | |
| Mouse IgG2a Negative Controlis suitable for use as a matched isotype negative control reagent to assess the level of non-specific binding of mouse IgG2a monoclonal antibodies to rat cells. This antibody has been tested and found to be negative on the following rat cells – peripheral blood leucocytes, thymocytes, splenocytes and macrophages.Mouse IgG2a Negative Control (MCA1210) recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product.THIS PRODUCT IS NOT SUITABLE FOR USE AS A NEGATIVE CONTROL ON HUMAN TISSUES. |
| 产品详情 | | |
Target Species | Negative Control |
Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide 1%Bovine Serum Albumin |
Immunogen | Human T lymphocytes. |
| Approx. Protein Concentrations | IgG concentration 0.1mg/ml |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | | * |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. *It is recommended that the user dilutes the antibody to a concentration equivalent to their test reagent.Use 10ul of the suggested working dilution to label 106cells in 100ul |
| 数据 |
| Figure A. PE conjugated mouse anti rat CD106 (MCA4633PE) and FITC conjugated mouse IgG2a isotype control (MCA1210F). Figure B. PE conjugated mouse anti rat CD106 (MCA4633PE) and FITC conjugated mouse anti rat CD11b (MCA619F). All experiments performed on red cell lysed rat bone marrow gated on mononuclear cells. |
| 文献 |
| 1. Fijak, M.et al.(2015) Influence of Testosterone on Inflammatory Response in Testicular Cells and Expression of Transcription Factor Foxp3 in T Cells.Am J Reprod Immunol. 74 (1): 12-25. |
技术参数 产品应用 Flow
产品类型 Negative/Isotype Control
推荐用于 Flow
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