NeuroG3 Recombinant Rabbit Monoclonal Antibody (10H35L14)

货号：703206

规格：100 µg

价格：4575

产品类型：免疫组化一抗

品牌：Thermo Fisher

物种：人/小鼠

宿主：兔

抗体亚型：IgG

克隆号：10H35L14

荧光染料：其它

类型:	单抗	同型对照：
浓度：	0.5 mg/mL	用法：	1:1,000 (WB)

产品详细信息This antibody is predicted to react with Monkey, Pig, Goat, Bovine.Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.靶标信息Neurog 3 protein contains one basic helix-loop-helix (bHLH) domain.It is both necessary and sufficient to induce endocrine islet cell differentiation from embryonic pancreatic progenitors. Not only does itinitiates endocrine cell differentiation, italso promotes islet cell maturation and maintains islet function. It is reported that Neurogenin 3 can redirect the differentiation of bipotential secretory progenitors to endocrine rather than goblet cell fate.

数据

NeuroG3 Antibody (703206) in WBWestern blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of MDAM-MB-231 (Lane 1), IMR-32 (Lane 2) and tissue extracts of Mouse Pancreas (Lane 3), Mouse Brain (Lane 4) and Mouse Pup Brain (Lane 5). The blot was probed with Anti-Neurogenin-3 Recombinant Rabbit Monoclonal Antibody (Product # 703206, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 30 kDa corresponding to Neurogenin-3 was observed in relevant cell lines and tissues tested.

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