H3K9acK14ac Polyclonal Antibody
货号：49-1010
规格：44 µg
价格：4809
产品类型：抗体和染料
品牌：Thermo Fisher
物种：人/小鼠/非人灵长类/猪/恒河猴
宿主：兔
抗体亚型：IgG
荧光染料：其它
| 类型: | 单抗 | 同型对照： | |
| 浓度： | | 用法： | 1:500 (WB)；1:200-1:250(ICC/IF)；1:150,000(ELISA)；2 µg (ChIP) |
靶标信息Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
| 数据 |
| H3K9acK14ac Antibody (49-1010) in ICC/IFImmunofluorescence analysis of Acetyl-Histone H3 (Lys9, Lys14) was performed using 70% confluent log phase HeLa cells treated with Trichostatin A (0.4uM, 18hr). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Acetyl-Histone H3 (Lys9, Lys14) Polyclonal Antibody (Product # 49-1010) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing increase in nuclear localization. Panel e represents control cells showing very weak nuclear expression . Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification. |
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| 参考文献： |
| 1.OncotargetCross-species genomic and epigenomic landscape of retinoblastoma."49-1010 was used in ChIP assay to analyze the cross-species genomic and epigenomic pattern of retinoblastoma" |
QQ:1749072012