CD73 Monoclonal Antibody (1D7)
货号：MA5-15537
规格：100 µL
价格：5463
产品类型：免疫组化一抗
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：1D7
荧光染料：其它
| 类型: | 单抗 | 同型对照： | |
| 浓度： | | 用法： | 1:500(WB)；1:200-1:1,000(IHC (P))；1:100 (ICC/IF)；1:10,000(ELISA) |
产品详细信息MA5-15537 targets NT5E in IHC applications and shows reactivity with Human samples.The MA5-15537 immunogen is purified recombinant fragment of NT5E expressed in E. Coli.MA5-15537 detects NT5E which has a predicted molecular weight of approximately 70kDa.靶标信息CD73 (Ecto-5-prime-nucleotidase, 5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. CD73 consists of a dimer of 2 identical 70 kDa subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. CD73 is used as a marker of lymphocyte differentiation. Consequently, a deficiency of CD73 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from CD73 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. The CD73 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations. Defects in the CD73 gene can lead to the calcification of joints and arteries, and intestinal tuberculosis. Two transcript variants encoding different isoforms of CD73 have been found.
| 数据 |
| CD73 Antibody (MA5-15537) in ICC/IFImmunofluorescence analysis of CD73 was performed using 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD73 Monoclonal Antibody (1D7) (Product # MA5-15537) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing membranous and cytosolic localization. The images were captured at 60X magnification. |
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