| 产品介绍 | | |
| Mouse anti Pig CD45, clone K252.1E4recognizes an epitope common to all porcine CD45 isoforms (Schnitzleinet al.1998). CD45 is also known as leukocyte common antigen (LCA).Mouse anti Pig CD45, clone K252.1E4 immunoprecipitates three polypeptides of 226, 210 and 190 kDa from preparations of porcine peripheral blood mononuclear cells and shows a broad reactivity pattern with both lymphoid and myeloid cells (Zuckermannet al.1994). |
| 产品详情 | | |
Target Species | Pig |
Product Form | Purified IgG- liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) |
| Carrier Free | Yes |
Immunogen | Porcine Peripheral Blood lymphocytes. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the P3 - X63 - Ag.653 myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/25 | 1/200 |
| Immunofluorescence | √ | | |
| Immunohistology - Frozen | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude itsuse in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106cells in 100ul. |
| 同型 |
| Description | Product Code | Applications | Pack Size |
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests/1ml |
| 文献 |
| 1. Antonson, A.M.et al.(2017) Maternal viral infection during pregnancy elicits anti-social behavior in neonatal piglet offspring independent of postnatal microglial cell activation.Brain Behav Immun. 59: 300-312.2. Westover, A.J.et al.(2016) An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome.J Diabetes Res. 2016: 3486727.3. Kaczmarek, M.M.et al.(2013) Seminal plasma affects prostaglandin synthesis and angiogenesis in the porcine uterus.Biol Reprod. 88 (3): 72.4. Valpotić, H.et al.(2018) Dietary supplementation with mannan oligosaccharide and clinoptilolite modulates innate and adaptive immune parameters of weaned pigs.Pol J Vet Sci. 21 (1): 83-93.5. Sauerova, P.et al.(2019) Positive impact of dynamic seeding of mesenchymal stem cells on bone-like biodegradable scaffolds with increased content of calcium phosphate nanoparticles.Mol Biol Rep. 46 (4): 4483-4500.6. McCleary, S.et al.(2020) Substitution of warthog NF-κB motifs into RELA of domestic pigs is not sufficient to confer resilience to African swine fever virus.Sci Rep. 10 (1): 8951.7. Zhang, W.et al.(2020) Dietary Calcium and Phosphorus Amounts Affect Development and Tissue-Specific Stem Cell Characteristics in Neonatal Pigs.J Nutr. 150 (5): 1086-92.8. Teuben, M.P.J.et al.(2021) Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence.Eur J Trauma Emerg Surg. Jun 10 [Epub ahead of print].9. McCormack, U.M.et al.(2019) Porcine Feed Efficiency-Associated Intestinal Microbiota and Physiological Traits: Finding Consistent Cross-Locational Biomarkers for Residual Feed Intake.mSystems.4 (4): e00324-18.10. Zimmermann, C.E.et al.(2021) Characterization of porcine mesenchymal stromal cells and their proliferative and osteogenic potential in long-term culture.J Stem Cells Regen Med. 17 (2): 49-55. |
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