产品介绍 | | |
| Mouse anti Human CD25, clone MEM-181recognizes the ~55 kDa alpha subunit of the human IL-2 receptor, also known as p55 or TAC antigen, CD25 is a type 1 transmembrane protein withtwo Sushi domains, also known as short concensus repeats (SCRs) or complement control protein (CCP) modules (Normanet al. 1991) located within its extracellular domain.The IL-2 receptor exists in three forms. A high affinity form consisting of a non-covalently linked heterodimer composed of the alpha subunit (CD25) and the IL-2 receptor beta subunit also known as CD122 or p75, a medium affinity beta subunit (CD122) monomer or a low affinity alpha (CD25) subunit monomer.CD25 is expressed by activated T lymphocytes and activated B lymphocytes responding to antigen or mitogen stimulation. CD25 is also expressed in some thymocytes and oligodendrocytes. In disease, elevated expression of CD25 in noted in a number of chronic inflammatory conditions, tuberculoid leprosy patients demonstrate markedly elevated levels of circulating CD25high FoxP3+ regulatory T cells (T-regs) (Attiaet al.2010).Elevated levels of CD25 antigen expression are often seen in cases ofnon-Hodgkin 's lymphomaand diffuse large B cell lymphoma (Fujiwaraet al.2013). |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG conjugated to StarBright Violet 475 - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A |
Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin0.1% Pluronic F680.1% PEG 33500.05% Tween 20 |
| Immunogen | Human PHA blasts; day 3 of culture. |
| Fusion Partners | Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line. |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| StarBright Violet 475 | 405 | 478 |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
存储条件 | | |
| Store at +4°C. DO NOT FREEZE.This product should be stored undiluted. |
应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | Neat | |
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.- Flow CytometryUse 5ul of the suggested working dilution to label 106cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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| 数据 |
| Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and StarBright Violet 475 conjugated Mouse anti Human CD25 (MCA2127SBV475). All experiments performed on human PBMCs stimulated with PHA for 3 days gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer. |
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| 文献 |
| 1. Zhang, Y.et al.(2013) Acceleratedin vivoproliferation of memory phenotype CD4+ T-cells in human HIV-1 infection irrespective of viral chemokine co-receptor tropism.PLoS Pathog. 9 (4): e1003310.2. Nocentini, G.et al.(2014) Expansion of regulatory GITR + CD25 Low/- CD4 + T cells in systemic lupus erythematosus patients.Arthritis Res Ther. 16: 444.3. Soukup, K.et al.(2015) The MAPK-Activated Kinase MK2 Attenuates Dendritic Cell-Mediated Th1 Differentiation and Autoimmune Encephalomyelitis.J Immunol. 195 (2): 541-52.4. Kusunoki, Y.et al.(2010) T-cell immunosenescence and inflammatory response in atomic bomb survivors.Radiat Res. 174 (6): 870-6.5. Bughani, U.et al.(2017) T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab.PLoS One. 12 (7): e0180088.6. Knutson, K.L.et al.(2015) Regulatory T cells, inherited variation, and clinical outcome in epithelial ovarian cancer.Cancer Immunol Immunother. 64 (12): 1495-504.7. Boland, J.W.et al.(2014) A preliminary evaluation of the effects of opioids on innate and adaptive humanin vitroimmune function.BMJ Support Palliat Care. 4 (4): 357-67.8. Luger, R.et al.(2013) Toll-like receptor 4 engagement drives differentiation of human and murine dendritic cells from a pro- into an anti-inflammatory mode.PLoS One. 8 (2): e54879.9. Rezalotfi, A.et al.(2020) Gastrospheres as a Model of Gastric Cancer Stem Cells Skew Th17/Treg Balance toward Antitumor Th17 Cells.J Immunol Res. 2020: 6261814.10. Thymianou, Set al. (2019) MBP7285 on Human Tcell ActivationMobile health Knoledge 21 Jul |
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