Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3、山羊抗大鼠 IgG (H+L)交叉吸附二抗, Cyanine3

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Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Cyanine3、山羊抗大鼠 IgG (H+L)交叉吸附二抗, Cyanine3

货号:A10522

规格:1mg

价格:2245

产品类型:荧光二抗

品牌:Thermo Fisher

物种:大鼠

宿主:山羊

抗体亚型:IgG

荧光染料:其它

类型:单抗同型对照:IgG
浓度:2 mg/mL用法:2 µg/mL(ICC);2 µg/mL (IF);1-10 µg/mL(IHC)
靶标信息抗大鼠第二抗体是具有亲和力的抗体,对大鼠免疫球蛋白具有良好的特异性,可用于检测,分选或纯化其特定靶标。 二抗提供了更多的多功能性,使用户能够使用许多检测系统(例如HRP,AP,荧光)。 它们还可以通过信号放大来提供更高的灵敏度,因为多个二级抗体可以与单个一级抗体结合。 最常见地,通过用来自靶物种的免疫球蛋白的汇集群体对宿主动物进行免疫来产生二抗,并且可以进一步纯化和修饰(即免疫亲和层析,抗体片段化,标记缀合等)以产生高度特异性的试剂。

数据

大鼠IF中的IgG(H + L)交叉吸附二抗(A10522)山羊抗大鼠IgG(H + L)二抗的免疫荧光分析是使用用α微管蛋白(YL1 / 2)大鼠单克隆抗体(产品编号MA1-80017)染色的A549细胞进行的。细胞用4%多聚甲醛固定10分钟,用0.1%Triton™X-100透化10分钟,用1%BSA封闭1小时,并在室温下用2μg/ mL大鼠一抗标记3小时。山羊抗鼠IgG(H + L)二抗Cy3(产品编号A10522)在室温下以2µg / mL的浓度在含0.2%BSA的磷酸盐缓冲液中使用,检测时间为45分钟,以检测细胞中的α微管蛋白。细胞质(面板a:红色)。在SlowFade®Gold Antifade Mountant(产品编号S36938)中,用DAPI将细胞核(面板b:蓝色)染色。 F-肌动蛋白用488 Phalloidin(产品编号A12379),1:300)(面板c:绿色)染色。面板d代表合成图像。单独的二抗(图f)或同种型对照(图e)未观察到非特异性染色。图像以60倍放大率拍摄。

推荐产品:

二抗

荧光二抗

免疫组化一抗

标签抗体

参考文献:

1.Breast cancer research : BCRImaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods."A10522 was used in immunohistochemistry - paraffin section to study the architecture of the mouse mammary gland using high-resolution 3D imaging"AuthorsLloyd-Lewis B,Davis FM,Harris OB,Hitchcock JR,Lourenco FC,Pasche M,Watson CJ2.Nature communicationsSingle-cell lineage tracing in the mammary gland reveals stochastic clonal dispersion of stem/progenitor cell progeny."A10522 was used in immunohistochemistry - paraffin section to examine the differentiation potential of adult mammary stem cells"AuthorsDavis FM,Lloyd-Lewis B,Harris OB,Kozar S,Winton DJ,Muresan L,Watson CJ3.Scientific reportsSurvival Motor Neuron (SMN) protein is required for normal mouse liver development."A10522 was used in immunohistochemistry - frozen section to provide evidence that the liver is an important therapeutic target for spinal muscular atrophy"AuthorsSzunyogova E,Zhou H,Maxwell GK,Powis RA,Muntoni F,Gillingwater TH,Parson SH4.The Journal of clinical investigationGq signaling causes glomerular injury by activating TRPC6."A10522 was used in immunohistochemistry - frozen section to develop and characterize a mouse model of glomerular disease"AuthorsWang L,Jirka G,Rosenberg PB,Buckley AF,Gomez JA,Fields TA,Winn MP,Spurney RF5.Developmental dynamics : an official publication of the American Association of AnatomistsStem cell marker prominin-1 regulates branching morphogenesis, but not regenerative capacity, in the mammary gland."A10522 was used in immunohistochemistry - frozen section to use a knockout model to determine the role of Prom1 in the mammary gland"AuthorsAnderson LH,Boulanger CA,Smith GH,Carmeliet P,Watson CJ
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