NGFR Human ELISA Kit/人NGFR ELISA试剂盒
货号:EHNGFR,EHNGFRX5,EHNGFRX10
规格:96 tests,5X96 tests,10X96 tests
价格:7311,29826,52892
产品类型:Platinum ELISA
品牌:Thermo Fisher
物种:人
宿主:其它
抗体亚型:其它
荧光染料:其它
灵敏度 | 80 pg/mL |
检测范围 | 0.082-20 ng/mL |
样本类型/体积 | 上清液 100 μL 血清 50 μL 血浆 50 μL |
操作时间 | 1 hr 20 min |
测试时间 | 4 hr 45 min |
Homogenous (no wash) | No |
批间变异系数(CV) | <12% |
批内变异系数(CV) | <10% |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 96 Tests |
组份 | - 预包被96孔板 - 标准品 - 分析稀释浓缩液 - 生物素化检测抗体 -SAV-HRP - 洗涤液 - 显色剂 - 终止液 - 胶板盖 |
储存温度 | 2-8℃ |
Protein name | NGFR (CD271) |
Protein aliases | CD271, Gp80-LNGFR, low affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, NGF receptor, p75 ICD, TNFR superfamily, member 16, Tumor necrosis factor receptor superfamily member 16 |
物种(Tested) | 人 |
试剂盒类型 | Sandwich ELISA Kit |
标记(染料) | HRP |
检测抗体偶联物 | Biotin |
Gene aliases | CD271, Gp80-LNGFR, NGFR, p75(NTR), p75NTR, TNFRSF16 |
Gene ID | (Human) 4804 |
Gene symbol | NGFR |
UniProt ID | (Human) P08138 |
The Human Nerve Growth Factor Receptor (Hu NGFR) ELISA quantitates Hu NGFR in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu NGFR.人神经生长因子受体(人NGFR)ELISA定量了人血清,血浆或细胞培养基中的人NGFR。 该检测试剂盒将专门识别天然和重组人NGFR。Principle of the method 方法原理The Human NGFR solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人NGFR固相夹心ELISA(酶联免疫吸附测定)设计用于测量匹配的抗体对之间结合的靶标量。 已在提供的微孔板的孔中预包被了靶标特异性抗体。 然后将样品,标准液或质控品添加到这些孔中,并与固定的(捕获)抗体结合。 通过添加第二(检测)抗体形成三明治,添加底物溶液,该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试,其标准包括敏感性,特异性,精密度和批次间一致性。 有关验证的更多信息,请参见手册。Target information 靶标信息Neurotrophic factors control the survival, differentiation and maintenance of neurons in the peripheral and central nervous systems, and of other neural crest-derived cell types. Developing sympathetic neurons are absolutely dependent upon nerve growth factor (NGF) during the period of target competition in vivo. Antibodies reacting specifically with NGFR p75 are useful tools in the detection and characterization of NGFR p75, to enhance our understanding of a wide range of phenomena in the development, plasticity and repair of the nervous system.神经营养因子控制周围和中枢神经系统以及其他神经c衍生细胞类型中神经元的存活,分化和维持。 在体内靶竞争期间,发展中的交感神经元绝对依赖于神经生长因子(NGF)。 与NGFR p75特异性反应的抗体是检测和鉴定NGFR p75的有用工具,可增进我们对神经系统发育,可塑性和修复过程中的理解。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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