Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eBioscience™

货号：14-4714-82,14-4714-81,14-4714-85

规格：100 µg,50 µg,500 µg

价格：1081,605,1567

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：P3.6.2.8.1

荧光染料：Purified

类型:	同型对照	同型对照：
浓度：	0.5 mg/mL	用法：	Assay-Dependent（Flow）； Assay-Dependent（IHC）; Assay-Dependent（ICC）; Assay-Dependent（IF）； Assay-Dependent（IP）

产品详细信息Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: This mouse IgG1 isotype control has been reported for use in surface and intracellular flow cytometric analysis, immunohistochemistry, immunocytochemistry, immunoprecipitation and immunoblotting (WB).Applications Tested: Mouse IgG1 K Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells and mouse spleen cells. It should be used at the same concentration as the experimental antibody.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.仅用于科研。不用于诊断过程。

数据

Mouse IgG1 kappa Isotype Control (14-4714-82) in IHC (P)Immunohistochemistry of formlin-fixed paraffin embedded human liver tissue using 1 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 1 µg/mL of Anti-Thrombospondin-1 Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin. Mouse IgG1 kappa Isotype Control (14-4714-82) in IHC (P)Immunohistochemistry of frozen mouse heart using 5 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 5 µg/mL of Anti-Mouse Connexin 43 Purified (right), followed by 10 µg/mL of F (ab')2 Anti-Mouse IgG eFluor® 570. Nuclei are stained with DAPI.

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参考文献：

1. Nature communicationsDynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory."14-4714 was used in Flow cytometry/Cell sorting to indicate the existence of a cardiac-specific trans-interacting chromatin domain functioning as a splicing factory."AuthorsBertero A,Fields PA,Ramani V,Bonora G,Yardimci GG,Reinecke H,Pabon L,Noble WS,Shendure J,Murry CEYear2019 2. JCI insightA TCRα framework-centered codon shapes a biased T cell repertoire through direct MHC and CDR3β interactions."14-4714 was used in Flow cytometry/Cell sorting to study the molecular basis underlying the selection of T cell receptor repertoires."AuthorsGunnarsen KS,Høydahl LS,Risnes LF,Dahal-Koirala S,Neumann RS,Bergseng E,Frigstad T,Frick R,du Pré MF,Dalhus B,Lundin KE,Qiao SW,Sollid LM,Sandlie I,Løset GÅYear2017Dilution1:160 3. Nature protocolsGenerating high-purity cardiac and endothelial derivatives from patterned mesoderm using human pluripotent stem cells."14-4714 was used in Flow cytometry/Cell sorting to develop a protocol which specifies cell fate efficiently into cardiac and endothelial lineages from human pluripotent stem cells."AuthorsPalpant NJ,Pabon L,Friedman CE,Roberts M,Hadland B,Zaunbrecher RJ,Bernstein I,Zheng Y,Murry CEYear2017Dilution1:100 4. PloS onePeptide-Based Optical uPAR Imaging for Surgery: In Vivo Testing of ICG-Glu-Glu-AE105."14-4714 was used in Flow cytometry/Cell sorting to develop a new uPAR-targeted fluorescent probe and evaluate its effectiveness in a human xenograft mouse model."AuthorsJuhl K,Christensen A,Persson M,Ploug M,Kjaer AYear2016 5. PloS oneFunctional proteomics screen enables enrichment of distinct cell types from human pancreatic islets."14-4714 was used in Flow cytometry/Cell sorting to discover marker combinations capable of enriching for discrete pancreatic cell subtypes from human pancreatic islets."AuthorsSharivkin R,Walker MD,Soen Y

技术参数

产品类型 细胞分选

产品线 EasySep™, RoboSep™

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