Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor 790/兔抗小鼠IgG（H + L）Superclonal™二抗，Alexa Fluor 790
货号：A27030
规格：1mg
价格：0
产品类型：荧光二抗
品牌：Thermo Fisher
抗原：Recombinant full-length protein
物种：小鼠
宿主：兔
抗体亚型：IgG
荧光染料：Alexa Fluor 790
| 类型: | 二抗 | 同型对照： | |
| 浓度： | 1 mg/mL | 用法： | 0.05-2 μg/mL (WB) |
产品详细信息The sensitivity and specificity of each lot is confirmed using ELISA.Minimal cross-reactivity with goat, rat, human, bovine, guinea pig, and donkey IgG is observed.Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds – the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody – recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets – a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
| 数据 |
| Mouse IgG (H+L) Secondary Antibody (A27030) in WBExpression of endogenous alpha-Tubulin was detected at 50 KDa using Mouse anti-alpha-Tubulin primary antibody (Product # 32-2500) (1:3000) and Rabbit anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 790 Conjugate (Product # A27030) (1 µg/mL 1:1000 dilution). Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa human cervical carcinoma cells (lane 1-3) using the Xcell Surelock Electrophoresis system and iBlot® Dry Blotting System. |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-17239.htm
QQ:1749072012