Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

货号：25-4714-42,25-4714-41

规格：100 Tests,25 Tests

价格：2037,1205

产品类型：小鼠同型对照

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：P3.6.2.8.1

荧光染料：PE-Cy7

类型:	同型对照	同型对照：
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test（Flow）； Assay-Dependent(Ctrl)

产品详细信息Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: PE-Cy7 Mouse IgG1, K Iso Cntrl has been reported for use in flow cytometric analysis.Applications Tested: This P3.6.2.8.1 antibody is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.仅用于科研。不用于诊断过程。

数据

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参考文献：

1.Oncology lettersAG490 reverses phenotypic alteration of dendritic cells by bladder cancer cells."25-4714 was used in Flow cytometry/Cell sorting to study the ability of AG490 to partly reverse the inhibitory effect of bladder cancer cells on dendritic cells, and induce their anti-tumour immunity response."AuthorsXiu W,Ma J,Lei T,Zhang MYear2018 2. Nature communicationsIL-21 drives expansion and plasma cell differentiation of autoreactive CD11chiT-bet+B cells in SLE."25-4714 was used in Flow cytometry/Cell sorting to describe and exemplify the importance of CD11chiT-bet+ B cells in human systemic lupus erythematosus."AuthorsWang S,Wang J,Kumar V,Karnell JL,Naiman B,Gross PS,Rahman S,Zerrouki K,Hanna R,Morehouse C,Holoweckyj N,Liu H,Manna Z,Goldbach-Mansky R,Hasni S,Siegel R,Sanjuan M,Streicher K,Cancro MP,Kolbeck R,Ettinger RYear2018 3. Breast cancer research : BCRThe hypoxic tumor microenvironment in vivo selects the cancer stem cell fate of breast cancer cells."25-4714 was used in Flow cytometry/Cell sorting to suggest that the hypoxic TME promotes malignant progression and therapy resistance via induction, selection and preferential maintenance of CSC-like cells."AuthorsKim H,Lin Q,Glazer PM,Yun ZYear20184. Dilution1:20 PloS oneCreation of an immunodeficient HLA-transgenic mouse (HUMAMICE) and functional validation of human immunity after transfer of HLA-matched human cells."25-4714 was used in Flow cytometry/Cell sorting to generate a novel immuno-deficient mouse strain expressing human HLA molecules instead of mouse MHC molecules."AuthorsZeng Y,Liu B,Rubio MT,Wang X,Ojcius DM,Tang R,Durrbach A,Ru Z,Zhou Y,Lone YCYear2017 5. Scientific reportsPlatelets confound the measurement of extracellular miRNA in archived plasma."25-4714 was used in Flow cytometry/Cell sorting to show that a single freeze/thaw cycle of plasma dramatically increases the number of platelet-derived MPs, contaminates the extracellular miRNA pool, and profoundly affects the levels of miRNAs detected."AuthorsMitchell AJ,Gray WD,Hayek SS,Ko YA,Thomas S,Rooney K,Awad M,Roback JD,Quyyumi A,Searles CDYear2016 6. RetrovirologyB cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity."25-4714 was used in Flow cytometry/Cell sorting to analyse HERV-H Env and HERV-W Env epitope expression on the surface of PBMCs from MS patients with active and stable disease."AuthorsBrudek T,Christensen T,Aagaard L,Petersen T,Hansen HJ,Møller-Larsen A

技术参数

产品应用 分化

细胞类型 Embryonic stem cells & iPS cells, Neural stem & progenitor cells, Neurons

产品线 STEMdiff™

适用物种 人

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