Mouse IgG2a kappa Isotype Control (eBM2a), eBioscience™

货号：14-4724-81,14-4724-82,14-4724-85

规格：50 µg,100 µg,500 µg

价格：605,1084,1575

产品类型：小鼠同型对照

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：eBM2a

荧光染料：Purified

类型:	同型对照	同型对照：
浓度：	0.5 mg/mL	用法：	Assay-Dependent（Flow）； Assay-Dependent(Ctrl)； Assay-Dependent（ICC）； Assay-Dependent（IHC）; Assay-Dependent（IF）

产品详细信息Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This mouse IgG2a isotype control has been reported for use in surface and intracellular flow cytometric analysis, immunocytochemistry, and immunohistochemistry.Applications Tested: This mouse IgG2a isotype control has been tested by flow cytometric analysis of human peripheral blood leukocytes and should be used at at the same concentration as the experimental antibody.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.仅用于科研。不用于诊断过程。

数据

Mouse IgG2a kappa Isotype Control (14-4724-81) in IHC (P)Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 5 µg/mL Mouse IgG2a K Isotype Control Purified (left) or 5 µg/mL Anti-Human CD20 Purified (Product # 14-0202-82) (right) followed by 10 µg/mL Anti-Mouse IgG2a Biotin and DAB visualization. Nuclei are counterstained with hematoxylin.Mouse IgG2a kappa Isotype Control (14-4724-81) in FlowStaining of a mixture of human iPSC and the C2C12 murine cell line with Anti-Human TRA-1-60 PerCP-eFluor 710 (Product # 46-8863-82) and 0.25 µg Mouse IgG2a K Isotype Control Purified (Product # 14-4724-82) (left) or 0.25 µg of Anti-Human CD321 (F11R) Purified (right) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4010-82).

推荐产品：

-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

文献：

1. Stem cell reportsDetection of Retinal Pigment Epithelium-Specific Antibody in iPSC-Derived Retinal Pigment Epithelium Transplantation Models."14-4724 was used in Flow cytometry/Cell sorting to determine if retinal pigment epithelium (RPE) transplantation could be performed using allografts, and whether this would produce RPE-specific antibodies."AuthorsSugita S,Makabe K,Fujii S,Iwasaki Y,Kamao H,Shiina T,Ogasawara K,Takahashi MYear2017 2. Scientific reportsFunctional dichotomy of Vδ2 γδ T cells in chronic hepatitis C virus infections: role in cytotoxicity but not for IFN-γ production."14-4724 was used in Flow cytometry/Cell sorting to analyse the phenotype and function of Vδ2 T cells in HCV-infected patients compared to healthy controls."AuthorsYin W,Tong S,Zhang Q,Shao J,Liu Q,Peng H,Hu H,Peng M,Hu P,Ren H,Tian Z,Zhang DYear2016 3. Journal of hematology and oncologyGenetically modified "obligate" anaerobic Salmonella typhimurium as a therapeutic strategy for neuroblastoma."14-4724 was used in Flow cytometry/Cell sorting to demonstrate the potential clinical therapeutic impact of targeting neuroblastoma by bio-engineered Salmonella with the assistance of macrophages."AuthorsGuo ZL,Yu B,Ning BT,Chan S,Lin QB,Li JC,Huang JD,Chan GCYear2015 4. Nature communicationsFucose-based PAMPs prime dendritic cells for follicular T helper cell polarization via DC-SIGN-dependent IL-27 production."14-4724 was used as a Control in experiments to demonstrate that carbohydrate-specific signalling by DC-SIGN drives follicular T helper cell differentiation via IL-27 expression."AuthorsGringhuis SI,Kaptein TM,Wevers BA,van der Vlist M,Klaver EJ,van Die I,Vriend LE,de Jong MA,Geijtenbeek TBYear2014 5. Journal of proteome researchPPE38 of Mycobacterium marinum triggers the cross-talk of multiple pathways involved in the host response, as revealed by subcellular quantitative proteomics."14-4724 was used in Western Blotting to determine proteome changes during the host response to the Mycobacterium marinum protein PPE38 in a murine macrophage cell line."AuthorsWang H,Dong D,Tang S,Chen X,Gao Q

技术参数

细胞类型 胚胎干细胞和iPS细胞，神经干细胞和祖细胞，神经元

产品线 STEMdiff™

适用物种 人

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-14521.htm