Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

货号：11-4714-41,11-4714-42

规格：25 Tests,100 Tests

价格：851,1383

产品类型：小鼠同型对照

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：P3.6.2.8.1

荧光染料：FITC

类型:	同型对照	同型对照：
浓度：	5 µL/Test	用法：	Assay-Dependent(ICC);Assay-Dependent(IF);Assay-Dependent(IHC);5 µL (1 µg)/test(Flow);Assay-Dependent(Ctrl)

产品详细信息Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: FITC Mouse IgG1 K Isotype Control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.Applications Tested: This Mouse IgG1 K Isotype Control is offered in 2 formats: - µg size: This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.仅用于科研。不用于诊断过程。

数据

Mouse IgG1 kappa Isotype Control (11-4714-42) in ICCImmunocytochemistry of fixed and permeabilized C6 cells using 1 µg/mL of Mouse IgG1 Isotype Control FITC (Product # 11-4714-42) (left) or 1 µg/mL of Anti-Vimentin FITC (right). Nuclei are counterstained with DAPI.

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参考文献：

1.Frontiers in pharmacologyDisruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma."11-4714 was used in Immunocytochemistry to indicate the enhanced anti-tumor efficacy of PD-1-deficient chimeric antigen receptor T cells against hepatocellular carcinoma."AuthorsGuo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z2.Frontiers in pharmacologyDisruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma."11-4714 was used in Immunocytochemistry to indicate the enhanced anti-tumor efficacy of PD-1-deficient chimeric antigen receptor T cells against hepatocellular carcinoma."AuthorsGuo X,Jiang H,Shi B,Zhou M,Zhang H,Shi Z,Du G,Luo H,Wu X,Wang Y,Sun R,Li Z3.Iranian journal of reproductive medicineComparison of differentiation potential of male mouse adipose tissue and bone marrow derived-mesenchymal stem cells into germ cells."11-4714 was used in Immunofluorescence to investigate appropriate sources beyond embryonic stem cells to obtain germ cells and to compare the differential potentials of these cells."AuthorsHosseinzadeh Shirzeily M,Pasbakhsh P,Amidi F,Mehrannia K,Sobhani A4.Frontiers in immunologyTGF-β Affects the Differentiation of Human GM-CSF+CD4+T Cells in an Activation- and Sodium-Dependent Manner."11-4714 was used in Flow cytometry/Cell sorting to study the environmental cues that promote differentiation of granulocyte-macrophage colony-stimulating factor producing T cells."AuthorsÉliás S,Schmidt A,Kannan V,Andersson J,Tegnér J5.Translational oncologyTumor-Penetrating Peptide Enhances Antitumor Effects of IL-24 Against Prostate Cancer."11-4714 was used in Flow cytometry/Cell sorting to show that interleukin-24 (IL-24)-iRGD induced apoposis and inhibited the growth of PC-3 cells to a significantly greater extent when compared with IL-24 treatment alone."AuthorsYang J,Yin H,Yang J,Wei Y,Fang L,Chai D,Zhang Q,Zheng J

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