ANTI-HUMAN CD38 (HB7) PE-Cyanine7
货号：25-0388-41,25-0388-42
规格：25TESTS,100TESTS
价格：1190,2996
产品类型：流式抗体
品牌：eBioscience
抗原：CD38
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：HB7
荧光染料：PE-Cy7
| 抗体类型: | 单抗 | 同型对照： | IgG1, kappa |
| 免疫原性： | | 用法： | 5uL（0.125 µg）/test(Flow） |
| Description: The HB7 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is an ectoenzyme which catalyses NAD into nicotinic acid adenine dinucleotide phosphate (NAADP) and cyclic ADP-ribose (cADPR), both of which are secondary messengers. Expression of CD38 is bimodal during B cell development, modulating from high in immature cells to low in intermediate ones and back to high on mature B cells. Additionally CD38 is found in a variety of tissues and other hematopoietic cells (e.g. T cells, NK cells and monocytes) and can be used to phenotype leukemias and monitor HIV-1 progression. The CD34+CD38- population of hematopoietic stems cells is thought to define the most pluripotent cells (e.g. blast colony forming cells). In addition to surface expression, CD38 has recently been found in the nucleus where it may play a role in monitoring calcium levels.Applications Reported: This HB7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HB7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD38 Antibody (25-0388-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or Anti-Human CD38 PE-Cyanine7 (purple histogram). Cells in the monocyte gate were used for analysis. |
| 推荐产品： |
| 造血干细胞HSC分选造血干细胞HSC扩增、分化培养基CD38流式抗体细胞因子 |
| 参考文献： |
| 1.Journal of cellular biochemistryCD38 is constitutively expressed in the nucleus of human hematopoietic cells.Authors Orciani M,Trubiani O,Guarnieri S,Ferrero E,Di Primio R2.BloodAnti-CD38-blocked ricin: an immunotoxin for the treatment of multiple myeloma.Authors Goldmacher VS,Bourret LA,Levine BA,Rasmussen RA,Pourshadi M,Lambert JM,Anderson KC3.BloodSequential generations of hematopoietic colonies derived from single nonlineage-committed CD34+CD38- progenitor cells.Authors Terstappen LW,Huang S,Safford M,Lansdorp PM,Loken MR4.Journal of immunology (Baltimore, Md. : 1950)Isolation of a cDNA encoding the human CD38 (T10) molecule, a cell surface glycoprotein with an unusual discontinuous pattern of expression during lymphocyte differentiation.Authors Jackson DG,Bell JI |
技术参数 产品应用 Flow；MISC
细胞类型 HSC
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