ALP碱性磷酸酶鉴定试剂盒

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ALP碱性磷酸酶鉴定试剂盒

货号:00-0055

规格:50 assays

价格:0

产品类型:干细胞鉴定

品牌:Stemgent

The undifferentiated state of embryonic stem (ES) and induced pluripotent stem (iPS) cells can be characterized by a high level of alkaline phosphatase (AP) expression which, along with the expression of other surface markers, indicates undifferentiated cells with the potential to self-renew.AP is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. When fixed ES or iPS cells are stained using the AP Staining Kit, undifferentiated cells appear red or purple, whereas differentiated cells appear colorless.

产品详情

Size50 assays
Kit ComponentsFix Solution: 25 ml (Cat. No. 09-0042)
AP Staining Solution A: 10 ml (Cat. No. 09-0043)
AP Staining Solution B: 10 ml (Cat. No. 09-0065)
AP Staining Solution C: 10 ml (Cat. No. 09-0066)
Storage and StabilityStore at 4°C. Do not freeze. Stable for 6 months from date of receipt when stored as directed.
Quality ControlThe AP Staining Kit II is functionally tested on human and mouse ES cells to ensure product quality.

参考文献

  1. Pease, S., Braghetta, P., Gearing, D., Grail, D., and Williams, R.L. (1990) Isolation of embryonic stem (ES) cells in media supplemented with recombinant leukemia inhibitory factor (LIF). Dev Biol. 141: 344-352.

  2. Smith, A.G., Nichols, J., Robertson, M., and Rathjen, P.D. (1992) Differentiation inhibiting activity (DIA/LIF) and mouse development. Dev. Biol. 151: 339-351.

  3. Takahashi, K., Tanabe K., Ohnuki, M., Narita, M., Ichisaka T., Tomoda K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131: 861-872.

  4. Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Nie, J., Jonsdottir, G.A., Ruotti, V., Stewart, R., Slukvin, I.I., and Thomson, J.A. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318: 1917-1920.

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