StainAlive™ SSEA-1 Antibody (DyLight™ 550), Mouse anti-Mouse/Human
货号：09-0095
规格：100 μl
价格：0
产品类型：流式抗体
品牌：Stemgent
物种：人
宿主：小鼠
抗体亚型：IgM
克隆号：MC-480
荧光染料：Purified
Stage-specific embryonic antigen-1 (SSEA-1), also known as CD15, is an antigenic epitope defined as a Lewis X carbohydrate structure. It is expressed on murine embryonal carcinoma (EC) cells, murine embryonic stem (ES) cells, and murine and human germ cells. SSEA-1 is widely used as a positive surface marker for mouse undifferentiated stem cells and a negative surface marker for human undifferentiated stem cells. Expression is down-regulated following differentiation of murine EC and ES cells, while the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression. SSEA-1 is associated with cell adhesion, migration, and differentiation. For use on live cells, this antibody has been specifically formulated with low endotoxin and no sodium azide. ES cell morphology, proliferation, and expression of pluripotency markers does not change after staining with this antibody.
产品详情
| Size | 100 μl |
| Concentration | 0.5 mg/ml |
| Clone | MC-480 |
| Isotype | Mouse IgM, κ |
| Immunogen | Mouse F9 teratocarcinoma stem cells (X-irradiated) |
| Reactivity | Mouse, Human |
| Preparation | This antibody was purified by affinity chromatography and conjugated with DyLight 550 under optimal conditions. The solution is free of unconjugated dye and unlabeled antibody. The formulation is 0.2 μm filter sterilized and free from bacteria, fungi, mycoplasma, and virus. |
| Formulation | Phosphate-buffered solution, pH 7.2, containing no preservative |
| Storage and Stability | Store at 4°C protected from light. Do not freeze. Handle under aseptic conditions. Stable for 6 months from date of receipt when stored as directed. |
| Quality Control | Tested by immunocytochemistry to ensure product quality. |
| Endotoxin level is less than 0.1 EU/μg (0.01 ng/μg) of the protein as determined by the LAL assay. |
| Recommended Usage | The suggested use of this antibody is a 1:100 dilution for immunocytochemistry. |
数据
ICC analysis of SSEA-1 Antibody on R1 mouse ES cells at 10X magnification. [A] Phase contrast view of cells in culture, [B] same field of view after staining with StainAlive SSEA-1 Antibody (DyLight 550) using a 1:100 dilution.
参考文献
Brambrink, T., Foreman, R., Welstead, G.G., Lengner, C.J., Wernig, M., Suh, H., and Jaenisch, R. (2008) Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2: 151-159.
Fox, N., Damjanov, I., Martinez-Hernandez, A., Knowles, B.B., and Solter, D. (1981) Immunohistochemical localization of the early embryonic antigen (SSEA-1) in postimplantation mouse embryos and fetal and adult tissues. Dev Biol 83: 391-398.
Andrews, P.W., Goodfellow, P.N., Shevinsky, L., Bronson, D.L., and Knowles, B.B. (1982) Cell surface antigens of a clonal human embryonic carcinoma cell line: morphological and antigenic differentiation in culture. Int. J. Cancer 29: 523-531.
Draper, J.S., Pigott, C., Thomson, J.A., and Andrews, P.W. (2002) Surface antigens of human embryonic stem cells: changes upon differentiation in culture. J. Anat. 200: 249-258.
Cui, L., Johkura, K., Yue, F., Ogiwara, N., Okouchi, Y., Asanuma, K., and Sasaki, K. (2004) Spatial distribution and initial changes of SSEA-1 and other cell adhesion-related molecules on mouse embryonic stem cells before and during differentiation. J Histochem Cytochem 52: 1447-1457.
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