货号：04-0012-02

规格：2 mg (10 mM)

价格：0

产品类型：小分子化合物

品牌：Stemgent

Y27632 is a cell-permeable small molecule Rho-associated kinase (ROCK) inhibitor¹. Y27632 has been found to prevent apoptosis as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency². This molecule has also been shown to enhance survival during the transplantation of ES cell-derived neural precursors³. Y27632 in combination with Pifithrin-µ significantly improves cell recovery after cryopreservation⁴.

Stemolecule Y27632 in Solution is a ready to use 10 mM stock solution for stem cell culture.

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Y27632 2 mg 04-0012

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参考文献

1. Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. (2000) Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57: 976-983.

2. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K., and Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells.Nat Biotechnol 25: 681-686.

3. Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. (2008) Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors.J. Neurosci Res. 86: 270-280.

4. Xu, X., Cowley, S., Flaim, C.J., James, W., Seymour, L.W., Cui, Z. (2010) Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation. Biotechnol Prog. 26: 781-788.