IMDM, HEPES/IMDM(含谷氨酰胺)

admin 2024-10-15 22

IMDM, HEPES/IMDM(含谷氨酰胺)

货号:01-058-1A

规格:500ml

价格:0

产品类型:细胞生物学

品牌:Stemgent

IMDM (Iscove's Modified Dulbecco's Medium) isutilized not only for more specialized cell types but also as the basis for some of the more unique serum-free media formulations.The original formulation designed by Iscove and associates, demonstrated that erythrocytes and macrophage precursor cells (e.g. primary Haemopoietic Cells) could be cultured in a totally defined serum-free medium when supplemented with albumin, lecithin, selenium and transferrin. IMDM is a modification of DMEM (Dulbecco's Modified Eagle's Medium) containing selenium, additional AA's, vitamins and other components. IMDM is a highly enriched synthetic media well-suited for rapidly proliferating and high-density cell cultures in a 5% CO2atmosphere.

IMDM medium reportedly supports Murine B Lymphocytes, Haemopoietic tissues from Bone Marrow, B-cells stimulated with Lipopolysaccharide,and also T-Lymphocytes and a wide variety of Hybrid cells. When utilized in conjunction with serum, it supports a broad spectrum of Mammalian cell types including but not exclusive to bone marrow, hybridomas and lymphocytes.

This IMDM is modified as follows:

With

  • L-Glutamine

  • Phenol Red

  • Sodium Bicarbonate

  • HEPES

Without:

  • Beta Mercaptoethanol

  • Alpha-Thioglycerol

Thecomplete formulationis available.

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