| 产品介绍 | | |
| Wnt-1, formerly known as int-1, is a non-glycosylated secreted protein associated with the extracellular matrix, involved in normal embryonic development and particularly linked with breast cancer.Wnt-1, which signals through binding to the Frizzled family of transmembrane receptors, was originally identified as a proto-oncogene implicated in carcinomas induced by the mouse mammary tumour virus (MMTV). Wnt-1 belongs to a family of Wnt genes involved in the dorso-ventricular development of the mesoderm during embryogenesis, with a restricted temporal and spatial pattern of expression. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified recombinant protein - lyophilized |
| Preparation | Purified recombinant Wnt-1 expressed inE.coli |
| Reconstitution | Reconstitute with 100 ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| Buffer Solution | 10mM Sodium Phosphate, pH6.8 |
| Activity | The ED50was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2. |
| Preservative Stabilisers | None present |
| Protein Molecular Weight | 38.4 kDa (343 amino acid residues) |
| Purity | >98% by SDS PAGE and HPLC |
| Regulatory | For research purposes only |
| Guarantee | 3 months from date of reconstitution |
| 存储条件 | | |
| Prior to reconstitution store at +4℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Functional Assays | √ | | |
| Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
QQ:1749072012