Crystalline Trypsin Solution (0.02%), without Phenol Red 水晶胰酶
货号：03-047-1A
规格：500 mL
价格：870
产品类型：细胞消化酶
品牌：BI
Product Overview
Crude trypsin is often the subculturing agent of choice for cell dissociation/ disaggregation of adherent cells, although the treatment may be cytotoxic if prolonged. Over-trypsinization is a common cause of subculture problems. When using crystalline trypsin versus crude trypsin forms, crystalline trypsin does not damage cells after prolonged exposure.
Advantages
Does not require multiple-change procedures
Maintains better cell viability and enhances the process of cell passaging
Not as cytotoxic to cells with all the negative ramifications of crude trypsin
Contains additives that protect the cell wall, enhancing cell viability, and it is a ready-to-use solution
| Storage Conditions | -20°C |
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| Specifications | Cells must NEVER remain in the crude trypsin for longer than 3-5 minutes as they may be seriously damaged in the process (i.e. damage to the intracellular proteins). Cells should NEVER be left without a fluid layer. The use of crystalline trypsin, rather than crude trypsin, most often performs better long-term cell growth in serum-free medium formulations. It is specifically formulated to have a gentle nature with much better cell viability, in which the cells are not subject to the vagaries of time and circumstance as when the cruder forms of trypsin are utilized.
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