Anti-Human TNF-Alpha APC

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Anti-Human TNF-Alpha APC

货号:84421-80

规格:100 tests

价格:3025

产品类型:抗体和染料

品牌:Biogems

物种:人

宿主:小鼠

抗体亚型:IgG

荧光染料:Purified

Catalogue Number :84421-80

Description

The MAb11 monoclonal antibody specifically reacts with human tumor necrosis factor alpha (TNF-alpha), a 157 amino acid non-glycosylated protein. TNF-alpha is a pleiotropic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-α is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation, and diabetes. The MAB11 antibody was generated from recombinant human TNF and is reported to be a neutralizing antibody.

Additional Information

Clone

MAb11

Format:

APC

Applications

FC

Reactivity

Human

Isotype:

Mouse IgG1, kappa

Research Interest:Innate Immunity, Cytokines & Growth Factors,Apoptosis
Cell Type:Th1 cells,Monocytes and Macrophages,NK and NKT Cells,T Cells

Preperation:

The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Formulation:

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

References:

Danis, V. A., Franic, G. M., Rathjen, D. A., & Brooks, P. M. (1991). Effects of granulocyte‐macrophage colony‐stimulating factor (GM‐CSF), IL‐2, interferon‐gamma (IFN‐gamma;), tumour necrosis factor‐alpha (TNF‐alpha;) and IL‐6 on the production of immunoreactive IL‐1 and TNF‐alpha; by human monocytes.Clinical & Experimental Immunology,85(1), 143-150.

Prussin, C., & Metcalfe, D. D. (1995). Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies.Journal of immunological methods,188(1), 117-128.

Andersen, H., Rossio, J. L., Coalter, V., Poore, B., Martin, M. P., Carrington, M., & Lifson, J. D. (2004). Characterization of rhesus macaque natural killer activity against a rhesus-derived target cell line at the single-cell level.Cellular immunology,231(1), 85-95.

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