GAPDH Loading Control Monoclonal Antibody (GA1R), HRP/GAPDH抗体

货号：MA5-15738-HRP

规格：50 µL

价格：2753

产品类型：内参抗体

品牌：Thermo Fisher

物种：人/小鼠/大鼠

宿主：小鼠

抗体亚型：IgG

荧光染料：HRP

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类型:	单抗	同型对照：	IgG1
免疫原性：	Recombinant GAPDH	用法：	1:500-2000(WB)

Product Specific InformationMA5-15738-HRP has been successfully used in Western blotting applications with yeast, bacteria, insect, human, mouse, rat, rabbit, hamster, and chicken samples.Background/Target InformationGAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer's disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington's disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

GAPDH Loading Control Antibody (MA5-15738-HRP) in WBWestern blot analysis of GAPDH was performed by loading 50 µg of various cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated GAPDH monoclonal antibody (Product # MA5-15738-HRP) at a dilution of 1:1000 overnight at 4°C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080).

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参考文献：

1.CellRAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses.View published figure onBy clicking the link above, you will be redirected to the Benchsci website.Disclaimer2.International journal of nanomedicineDiamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion.View published figure onBy clicking the link above, you will be redirected to the Benchsci website.Disclaimer3.Scientific reportsLow Frequency Magnetic Fields Induce Autophagy-associated Cell Death in Lung Cancer through miR-486-mediated Inhibition of Akt/mTOR Signaling Pathway.View published figure onBy clicking the link above, you will be redirected to the Benchsci website.Disclaimer4.AutophagyProgress of endocytic CHRN to autophagic degradation is regulated by RAB5-GTPase and T145 phosphorylation of SH3GLB1 at mouse neuromuscular junctions in vivo.View published figure onBy clicking the link above, you will be redirected to the Benchsci website.Disclaimer5.American journal of physiology. Endocrinology and metabolismZinc finger protein 407 overexpression upregulates PPAR target gene expression and improves glucose homeostasis in mice.View published figure onBy clicking the link above, you will be redirected to the Benchsci website.Disclaimer

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