CFSE
货号：63310-00
规格：500ug
价格：880
产品类型：细胞染料
品牌：Biogems
物种：其它
宿主：其它
抗体亚型：其它
荧光染料：Purified
CFSE是用于细胞示踪、增殖和细胞运动研究的试剂。CFSE被动扩散进入细胞后，由于其乙酸酯基团被细胞内酯酶切割，被转化为荧光羧基荧光素分子。因CFSE分子与细胞内蛋白质形成稳定的共价交联，所以荧光可长时间保留在细胞内。当细胞分裂时，CFSE荧光强度会随细胞分裂而减半。CFSE 激发波为494nm，发射波为521nm，并且可以与含有甲醛和皂苷的透膜缓冲液的标准固定剂一起使用。
CFSE is a reagent useful for cell tracking, proliferation studies, and cell motility studies. After passively diffusing into cells, it is converted to a fluorescent carboxyfluorescin molecule as its acetate groups are cleaved by intracellular esterases. The fluorescent CFSE is retained inside the cell for extended periods, because the molecules forms stable covalent crosslinks with the intracellular proteins. At each cell division, the intensity of the CFSE fluorescence is halved. The peak excitation of the molecule is 494 nm and the peak emission is 521 nm and it may be used with standard fixatives containing formaldehyde and permeabilization buffers that contain saponin.
Additional Information
Applications | FC |
Application Notes: | CFSE has been tested by flow cytometric analysis of stimulated human peripheral blood mononuclear cells. It can be used at concentrations ranging from 0.5 to 20 uM for cell labeling. For optimal performance, it is recommended that the used concentration be empirically determined for the assay of interest. |
Formulation: | Lyophilized |
References: | Ingulli, E. (2007). Tracing tolerance and immunity in vivo by CFSE-labeling of administered cells. InImmunological Tolerance(pp. 365-376). Humana Press. Lyons, A. B., & Parish, C. R. (1994). Determination of lymphocyte division by flow cytometry.Journal of immunological methods,171(1), 131-137. Parish, C. R., Glidden, M. H., Quah, B. J., & Warren, H. S. (2009). Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation.Current protocols in immunology, 4-9. |
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