CD3e Monoclonal Antibody (eBio500A2 (500A2)), PE, eBioscience™

货号：12-0033-82,12-0033-85

规格：100 µg,500 µg

价格：1416,4051

产品类型：流式抗体

品牌：eBioscience

抗原：CD3e

物种：小鼠

宿主：其它

抗体亚型：IgG

克隆号：eBio500A2 (500A2)

荧光染料：PE

类型:	一抗	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™
浓度：	0.5 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.Applications Reported: The 145-2C11 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components havelong cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.仅用于科研。不用于诊断过程。

数据

CD3e Antibody (12-0033-82) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.06 µg of Golden Syrian Hamster IgG Isotype Control PE (Product # 12-4914-81) (left) or 0.06 µg of Anti-Mouse CD3e PE (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Acta neuropathologica communicationsSurfen, a proteoglycan binding agent, reduces inflammation but inhibits remyelination in murine models of Multiple Sclerosis."12-0033 was used in Flow cytometry/Cell sorting to study surfen, an agent that binds both heparan sulphate proteoglycans and chondroitin sulphate proteoglycans."AuthorsWarford JR,Lamport AC,Clements DR,Malone A,Kennedy BE,Kim Y,Gujar SA,Hoskin DW,Easton ASYear2018SpeciesMouse 2.Nature communicationsFat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation."12-0033 was used in Flow cytometry/Cell sorting to reveal a link between inflammation, IL-33 release by FALC stromal cells, ILC2 activation and pleural B-cell activation in FALCs, resulting in local and antigen-specific IgM production."AuthorsJackson-Jones LH,Duncan SM,Magalhaes MS,Campbell SM,Maizels RM,McSorley HJ,Allen JE,Bénézech CYear2016SpeciesMouseDilution1:200 3.Journal of hematology & oncologyAzacytidine mitigates experimental sclerodermic chronic graft-versus-host disease."12-0033 was used in Flow cytometry/Cell sorting to assessed the impact of azacytidine on cGVHD in a murine model of sclerodermic cGVHD."AuthorsFransolet G,Ehx G,Somja J,Delens L,Hannon M,Muller J,Dubois S,Drion P,Caers J,Humblet-Baron S,Delvenne P,Beguin Y,Conteduca G,Baron FYear2016SpeciesMouse 4.British journal of cancerGemcitabine enhances the efficacy of reovirus-based oncotherapy through anti-tumour immunological mechanisms."12-0033 was used in Flow cytometry/Cell sorting to demonstrate that reovirus and gemcitabine combination treatment postpones peritoneal carcinomatosis development and prolongs the survival of cancer-bearing hosts."AuthorsGujar SA,Clements D,Dielschneider R,Helson E,Marcato P,Lee PWYear2014SpeciesMouse 5.Journal of immunology (Baltimore, Md. : 1950)Y chromosome-linked B and NK cell deficiency in mice."12-0033 was used in Flow cytometry/Cell sorting to establish a novel mouse strain in which all males lack B and natural killer cells and have Peyer's patch defects."AuthorsSun SL,Horino S,Itoh-Nakadai A,Kawabe T,Asao A,Takahashi T,So T,Funayama R,Kondo M,Saitsu H,Matsumoto N,Nakayama K,Ishii N

技术参数

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