CD27 Monoclonal Antibody (O323), FITC, eBioscience™

货号：11-0279-42

规格：100 Tests

价格：1886

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号： O323

荧光染料：FITC

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC
浓度：	5 µL/Test	用法：	5 μL (1 μg)/test(Flow)

产品详细信息Description: The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.Applications Reported: The O323 antibody has been reported for use in flow cytometric analysis.Applications Tested: The O323 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

数据

CD27 Antibody (11-0279-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (left) or Anti-Human CD27 FITC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.OncoimmunologyAntigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities."Published figure using CD27 monoclonal antibody (Product # 11-0279-42) in Flow Cytometry"2.Journal of leukocyte biologyPerturbed CD8+T cell immunity across universal influenza epitopes in the elderly."Published figure using CD27 monoclonal antibody (Product # 11-0279-42) in Flow Cytometry"3.Experimental and therapeutic medicineAbnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection."Published figure using CD27 monoclonal antibody (Product # 11-0279-42) in Flow Cytometry"4.Immunology and cell biologyMaintenance of the EBV-specific CD8+TCRαβ repertoire in immunosuppressed lung transplant recipients."Published figure using CD27 monoclonal antibody (Product # 11-0279-42) in Flow Cytometry"5.Nature communicationsA human immunodeficiency syndrome caused by mutations in CARMIL2."11-0279 was used in Flow cytometry/Cell sorting to show that human CARMIL2-deficiency is associated with defective CD28-mediated TCR co-signalling and impaired cytoskeletal dynamics."AuthorsSchober T,Magg T,Laschinger M,Rohlfs M,Linhares ND,Puchalka J,Weisser T,Fehlner K,Mautner J,Walz C,Hussein K,Jaeger G,Kammer B,Schmid I,Bahia M,Pena SD,Behrends U,Belohradsky BH,Klein C,Hauck F

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