Leptin Human Instant ELISA Kit/人瘦素一步法ELISA试剂盒
货号：BMS2039INST
规格：128 tests
价格：6668
产品类型：Instant ELISA
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度： | 20.0 pg/mL | 产品规格： | 128 tests |
检测范围： | 63-4,000 pg/mL | 标记染料： | HRP |
仪器： | 比色酶标仪 | 储存： | -20°C |
操作时间： | 20 min | 蛋白名称： | Leptin |
测试时间： | 3 hr 10 min | 特异性靶标位点： | |
一致性（未洗涤）： | No | 物种（经过测试）： | Human |
批间变异系数： | 6.9% | 检测试剂盒类型： | 即用型ELISA 试剂盒 |
批内变异系数： | 5.7% | 检测抗体偶联物： | Biotin |
| 同名基因： | LEPD, OB, OBS | 基因名称： | LEP |
样品类型/体积： | 血清 10 µL 血浆 10µL | 组分： | - 预包被96孔板 - 生物素化检测抗体 - 链霉亲和素-辣根过氧化物酶 - 样品稀释液，冻干 - 标准曲线条 - 样品稀释液 - 洗涤浓缩液 - 底物溶液 - 终止液 - 裂解液10X - 胶板盖 |
| The Human Leptin ELISA quantitates Hu Leptinin human serum, plasma or other body fluids. The assay will exclusively recognize both natural and recombinant Hu Leptin.人Leptin ELISA可以定量人瘦素在人的血清，血浆或其他体液中的含量。 该检测试剂盒将专门识别天然和重组Hu Leptin。Principle of the method 方法原理The Human Leptin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人Leptin夹心ELISA（酶联免疫吸附测定）是测量匹配的抗体对之间结合的靶标量。 已在孔中预包被了靶标特异性抗体。 将样品或对照添加到这些孔中，并与固定的（捕获）抗体结合。 通过将第二（检测）抗体与捕获抗体不同表位上的靶标结合形成三明治。 与酶结合的抗体结合形成的三明治。 经过孵育和洗涤步骤以去除微孔板中未结合的物质后，添加底物溶液，该溶液与酶-抗体-靶标复合物反应以产生可测量的信号。 该信号的强度与原始样品中存在的靶标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。 有关验证的更多信息，请参见手册。Target information 靶标信息Leptin is a 167 amino acid long protein hormone with important effects in regulating body weight, metabolism and reproductive function. Leptin is approximately 16 kDa in mass and encoded by the obese (ob) gene. Leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cells in the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. Leptin's effects on body weight are mediated through effects on hypothalamic centers that control feeding behavior and hunger, body temperature and energy expenditure. Leptin is involved in regulating food intake, energy expenditure, and adiposity through hypothalamic leptin receptors. Leptin promotes hematopoiesis, angiogenesis, wound healing, inflammation, immune responses, influences pubertal development and fetal growth. Studies have investigated the role of leptin in obesity, anorexia nervosa, insulin resistance, and hypertension. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production.Leptin是一种167个氨基酸的长蛋白激素，对调节体重，代谢和生殖功能具有重要作用。瘦蛋白的质量约为16 kDa，由肥胖（ob）基因编码。瘦素主要由脂肪细胞表达，这与将体重视为体内脂肪的总质量的想法相吻合。胃上皮细胞和胎盘中的细胞也会分泌少量的瘦素。瘦素受体在下丘脑的区域中高表达，已知对调节体重很重要，在T淋巴细胞和血管内皮细胞中也是如此。瘦素对体重的影响通过对下丘脑中枢的影响来介导，该下丘脑中枢控制进食行为和饥饿，体温和能量消耗。瘦素通过下丘脑瘦素受体参与调节食物摄入，能量消耗和肥胖。瘦素促进造血，血管生成，伤口愈合，炎症，免疫反应，影响青春期发育和胎儿生长。研究已经研究了瘦素在肥胖，神经性厌食症，胰岛素抵抗和高血压中的作用。瘦素还具有生热作用并调节脂肪酸氧化酶。瘦蛋白生产中的缺陷可能导致啮齿动物和人类中的严重遗传性肥胖。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
| 特点： |
| -极其简便快捷：操作时间仅15 min- 使用率更高：单独的标品板条，可检测96个样品- 适用客户：高度追求实验效率的客户 |
| 数据： |
Leptin Human Instant ELISA™ KitRepresentative standard curve for Human Leptin Instant ELISA |
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