Triton™ X-100

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Triton™ X-100

货号:T8787-100ML

规格:100mL

价格:397.8

产品类型:化学试剂

品牌:Sigma

Triton X-100 is a non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. Triton X-100 has no antimicrobial properties. It is considered as a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It does absorb in the ultraviolet region of the spectrum, however, so can interfere with protein quantitation.[4][5][6]

应用

- To permeabilise cells during Immunofluorescent microscopic studies[1]- In immunohistochemistry for staining the Flat-mount retinas[2]- For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure[3]

性质
Related CategoriesAntigen-Vaccine Preparation,Apoptosis and Cell Cycle,Biochemicals,Cell Culture,Core Bioreagents,

更多...

grade

for molecular biology

description

non-ionic

InChI Key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

form

liquid

mol wt

micellar avg mol wt 80,000

average mol wt 625

aggregation number

100-155

CMC

0.2-0.9mM(20-25°C)

transition temp

cloud point 65°C

pour point ~7°C

solubility

water: 0.1mL/mL clear to slightly hazy, colorless to faintly yellow

density

1.06g/mLat 25°C(lit.)

HLB

13.5

foreign activity

DNase and RNase, none detected

参考文献

1. Sealing the skin barrier around transcutaneous implants: in vitro study of keratinocyte proliferation and adhesion in response to surface modifications of titanium alloy.

2. Ischemic neurons prevent vascular regeneration of neural tissue by secreting semaphorin 3A.

3. Effects of four different single exercise sessions on lipids, lipoproteins, and lipoprotein lipase.

4. Nondetergent isolation of rafts.

5. Mammalian long-chain acyl-CoA synthetases.

6. The isolation of detergent-resistant lipid rafts for two-dimensional electrophoresis.

技术参数
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