Triton™ X-100
货号:T8787-100ML
规格:100mL
价格:397.8
产品类型:化学试剂
品牌:Sigma
Triton X-100 is a non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. Triton X-100 has no antimicrobial properties. It is considered as a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It does absorb in the ultraviolet region of the spectrum, however, so can interfere with protein quantitation.[4][5][6]
应用
- To permeabilise cells during Immunofluorescent microscopic studies[1]- In immunohistochemistry for staining the Flat-mount retinas[2]- For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure[3]
性质
Related Categories | Antigen-Vaccine Preparation,Apoptosis and Cell Cycle,Biochemicals,Cell Culture,Core Bioreagents, 更多... |
grade | for molecular biology |
description | non-ionic |
InChI Key | HNLXNOZHXNSSPN-UHFFFAOYSA-N |
form | liquid |
mol wt | micellar avg mol wt 80,000 |
| average mol wt 625 |
aggregation number | 100-155 |
CMC | 0.2-0.9mM(20-25°C) |
transition temp | cloud point 65°C |
| pour point ~7°C |
solubility | water: 0.1mL/mL clear to slightly hazy, colorless to faintly yellow |
density | 1.06g/mLat 25°C(lit.) |
HLB | 13.5 |
foreign activity | DNase and RNase, none detected |
参考文献
1. Sealing the skin barrier around transcutaneous implants: in vitro study of keratinocyte proliferation and adhesion in response to surface modifications of titanium alloy.
2. Ischemic neurons prevent vascular regeneration of neural tissue by secreting semaphorin 3A.
3. Effects of four different single exercise sessions on lipids, lipoproteins, and lipoprotein lipase.
4. Nondetergent isolation of rafts.
5. Mammalian long-chain acyl-CoA synthetases.
6. The isolation of detergent-resistant lipid rafts for two-dimensional electrophoresis.
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