Pierce High Capacity Streptavidin Chromatography Cartridges, 1 mL 高容量链霉亲和素色谱柱，1 mL
货号：87739,87740
规格：2 x 1 mL,1 x 5 mL
价格：3789,5168
产品类型：蛋白提取/纯化
品牌：Thermo Fisher
| The Thermo Scientific Pierce High Capacity Streptavidin Chromatography Cartridges are convenient, ready-to-use devices for separating biotinylated molecules from non-biotinylated molecules and for purifying antigens using biotinylated antibodies. The chromatography cartridges contain a beaded resin of immobilized recombinant streptavidin protein that binds biotinylated proteins at greater than 10 mg/mL of beads. This corresponds to 2 to 3 times higher biotin-binding capacity than products from other suppliers and is ideal when a high amount of recovery is required.These cartridges enable fast, easy, and reproducible chromatographic separations and are compatible with the major automated liquid-chromatography systems or manual syringe processing (see Table 1 for cartridge general properties). The cartridges attach directly to ÄKTA™ or FPLC systems without additional connectors. The included accessory pack readily adapts cartridges for use with Luer-Lok syringe fittings or 1/16" tubing. These cartridges can be regenerated multiple times for affinity purification.Pierce High Capacity Streptavidin Chromatography Cartridges consist of purified recombinant streptavidin covalently immobilized at a high density onto high-quality cross-linked 6% beaded agarose. Biotinylated antibodies, proteins, peptides, nucleic acids, and other molecules or interaction complexes can be captured, immunoprecipitated, removed, or purified from samples using this streptavidin resin.Streptavidin is a tetrameric protein containing four biotin-binding sites similar to avidin. The native protein from Streptomyces avidinii is carbohydrate-free, has an acidic pI of 5.5 and a mass of 75 kDa. The recombinant protein used to manufacture Pierce streptavidin resins has a near-neutral isoelectric point and a mass of 53 kDa. Streptavidin generally has less nonspecific binding than avidin (from chicken egg white) because of the absence of carbohydrates. |
| 特点： |
| - Streptavidin—purified recombinant streptavidin protein (53 kDa, near-neutral pI); tetrameric with four biotin-binding site per molecule- Agarose resin—support is cross-linked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods- Inert and stable—streptavidin is immobilized by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield- High capacity—this variety of beads has a dense load of immobilized streptavidin, providing a binding capacity greater than 10 mg of biotinylated BSA per mL of resin- Two formats—available in several bottle sizes of resin slurry and as packed 1 mL and 5 mL chromatography cartridges |
| 数据： |
Dimensions of the Pierce Chromatography Columns Properties of the Pierce High Capacity Streptavidin Chromatography Cartridges |
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