OneTaq® 2X Master Mix with Standard Buffer
货号：M0482S
规格：100 reactions (50 μl vol)
价格：0
产品类型：PCR 及DNA聚合酶
品牌：NEB
The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification (1). Taq DNA Polymerase is an enzyme widely used in PCR (2). The following guidelines are provided to ensure successful PCR using New England Biolabs’ OneTaq 2X Master Mix with Standard Buffer. These guidelines cover routine PCR. Amplification of templates with high GC content, high secondary structure or low template concentrations may require further optimization.
技术参数 产品优点 Application Features： GC-rich PCR High Sensitivity PCR High Throughput PCR Colony PCR Long PCR (up to ~6 kb genomic) AT-rich PCR
产品应用 Routine and difficult PCR experiments
组成成分 1X Master Mix Composition20 mM Tris-HCl 0.06% IGEPAL® CA-630 5% Glycerol 0.2 mM dNTPs 25 units/ml OneTaq® DNA Polymerase 0.05% Tween® 20 1.8 mM MgCl2 22 mM KCl 22 mM NH4Cl pH 8.9@25°C
辅助产品 Magnesium Chloride (MgCl2) Solution OneTaq® DNA Polymerase OneTaq® Hot Start DNA Polymerase OneTaq® 2X Master Mix with GC Buffer OneTaq® Quick-Load® 2X Master Mix with Standard Buffer OneTaq® Hot Start 2X Master Mix with Standard Buffer
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