Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP/山羊抗小鼠IgG (H+L)二抗，HRP

货号：32430

规格：2 mL

价格：2333

产品类型：二抗

品牌：Thermo Fisher

抗原：Purified Mouse IgG, whole molecule

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：HRP

抗体类型:	荧光二抗	同型对照：
浓度：	0.01 mg/mL	用法：	1:60-1:500（ELISA）； 1:6-1:60 (IHC)； 1:10,000 (IP);1:60-1:500 (WB)

产品详细信息

This antibody may cross-react with immunoglobulins from other species.Working dilutions for Western blotting using SuperSignal West Substrates:SuperSignal West Pico Substrate - 1:250-1:1250SuperSignal West Dura Substrate - 1:600-1:3000SuperSignal West Femto Substrate - 1:1250-1:6000ECL Western Blotting Substrate - 1:20-1:125靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Secondary Antibody (32430) in WBWestern blot analysis of STAT3 was performed by loading 25 µg of U2-OS lysate from STAT3 SMART pool siRNA (Product # L-003544-00-0005) transfected or non-targeting control (Product # D-001810-10) transfected U2-OS cells onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with STAT3 (Clone 9D8) mouse monoclonal antibody at a dilution of 1:1000 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Dura (Product # 34075). Mouse IgG (H+L) Secondary Antibody (32430) in WBWestern blot analysis of HA Epitope Tag was performed by various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HA Epitope Tag monoclonal antibody (Product # 26183) at a dilution of 1:10,000 for 1 hour at room temperature on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). Mouse IgG (H+L) Secondary Antibody (32430) in WBWestern blot analysis of CREB was performed by loading 25 µg of various whole cell lysates onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% Milk/TBST for at least 1 hour. Membranes were probed with a mouse monoclonal antibody recognizing CREB (Product # MA1-083) at a dilution of 1:500 overnight at 4°C on a rocking platform. Membranes were washed in TBS-0.1%Tween 20 and probed with a goat anti-mouse-HRP secondary antibody (Product # 32430) at a dilution of 1:20,000 for at least one hour. Membranes were washed and chemiluminescent detection performed using Pierce Super Signal West Pico (Product # 34077).

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参考文献：

1. Human molecular geneticsRescue of mutant rhodopsin traffic by metformin-induced AMPK activation accelerates photoreceptor degeneration.2. Journal of molecular and cellular cardiologyTranscriptional regulation of the sodium channel gene (SCN5A) by GATA4 in human heart.3. Journal of cell scienceProlyl endopeptidase is involved in the degradation of neural cell adhesion molecules in vitro.4. Journal of cell scienceAn unrecognized function for COPII components in recruiting the viral replication protein BMV 1a to the perinuclear ER.5. Journal of virological methodsIdentification of an epitope within the Bovine herpesvirus 1 glycoprotein E cytoplasmic tail and use of a monoclonal antibody directed against the epitope for the differentiation between vaccinated and infected animals.

技术参数

产品应用 ELISA;IHC;IP;WB

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