OneTaq® 2X Master Mix with GC Buffer
货号：M0483S
规格：100 reactions (50 μl vol)
价格：0
产品类型：PCR 及DNA聚合酶
品牌：NEB
OneTaq® 2X Master Mix with GC Buffer is an optimized blend of Taq and Deep VentR™ DNA Polymerases ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies, and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient Master Mix formulation contains dNTPs, MgSO4, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.
技术参数 产品优点 OneTaq® 2X Master Mix with GC Buffer is an optimized blend of Taq and Deep VentR™ DNA Polymerases ideally suited to PCR applications from GC-rich templates, including pure DNA solutions, bacterial colonies, and cDNA products. The 3´→5´ exonuclease activity of Deep Vent DNA Polymerase increases the fidelity and robust amplification of Taq DNA Polymerase (1). The convenient Master Mix formulation contains dNTPs, MgSO4, buffer components and stabilizers, requiring only the addition of primers and DNA template for robust amplification.
产品应用 Application FeaturesGC-rich PCR High Sensitivity PCR High Throughput PCRColony PCR Long PCR (up to ~6 kb genomic)
组成成分 1X Master Mix Composition0.06% IGEPAL® CA-630 0.05% Tween® 20 20 mM (NH4)2SO4 2 mM MgSO4 5% Glycerol 80 mM Tris-SO4 5% DMSO 0.2 mM dNTPs 25 units/ml OneTaq® DNA Polymerase pH 9.2@25°C
辅助产品 Companion Products Magnesium Sulfate (MgSO4) Solution OneTaq® DNA Polymerase OneTaq® Hot Start DNA Polymerase OneTaq® 2X Master Mix with Standard Buffer OneTaq® Quick-Load® 2X Master Mix with Standard Buffer OneTaq® Quick-Load® 2X Master Mix with GC Buffer
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