货号：108919

规格：25 µg

价格：1260

产品类型：流式抗体

品牌：Biolegend

物种：小鼠

宿主：仓鼠

抗体亚型：IgM

荧光染料：APC

Description

DX5 antigen has been recently characterized as CD49b. It is a 150 kD integrin α chain also known as α2integrin, VLA-2 α chain, and integrin α2chain. CD49b non-covalently associates with CD29 (β1integrin) to form the CD49b/CD29 complex known as VLA-2, a receptor for collagen and laminin. CD49b is expressed on platelets, the majority of NK cells, NKT cells, and a small subset of CD8+ T cells (this population can be significantly increased following viral infection). DX5 is used for the identification and isolation of NK cells, and is especially useful for identifying NK cells in mice lacking the NK1.1 antigen.

• Reactivity

• Mouse

• Immunogen

• IL-2-propagated NK1.1+cells from C57BL/6 mice

• Formulation

• Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

• Preparation

• The antibody was purified by affinity chromatography and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.

• Concentration

• 0.2 mg/ml

• Storage & Handling

• The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.

• Application

• FC - Quality tested

• Recommended Usage

• Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

• Excitation Laser

• Red Laser (633 nm)

• Application Notes

• The DX5 clone detects cells expressing relatively high levels of CD49b and may not be useful for the detection of cells expressing low levels of CD49b. DX5 does not block NK cell killing or binding to collagenin vitro.Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity2and immunohistochemical staining5of formalin-fixed and paraffin-embedded tissue sections as well as immunohistochemical staining of acetone-fixed frozen sections10. The binding of DX5 antibody to splenic NK cells can be blocked by HMα2 antibody.

• Application References(PubMed link indicates BioLegend citation)

• 1. Arase H,et al.2001.J. Immunol.167:1141. (FC)2. Sepulveda H,et al.1999.J. Immunol.163:1133.3. Norian LA and Allen PM. 2004.J. Immunol.173:835. (FC)4. Andoniou CE,et al.2005.Nature Immunology6:1011.5. Oertelt S,et al.2006.J. Immunol.177:1655. (IHC)PubMed6. Bourdeau A,et al.2007.Blooddoi:10.1182/blood-2006-08-044370.7. Charles N,et al.2010.Nat. Med.16:701. (FC)PubMed8. Qui Q,et al.2010.J. Immunol.184:1681. (FC)PubMed9. Busche A,et al.2011.J. Immunol.186:2918.PubMed10. Kim HR,et al.2011.Nephrology16:545. (IHC)PubMed11. Seyoum B,et al.2011.Vaccine.29:8002.PubMed12. Younos IH,et al.2012.Int Immunopharmacol.13:245.PubMed13. Honjo K,et al.2012.PNAS.PubMed14. Gough DJ,et al.2014.Blood.124:2252.PubMed