货号：R2062

规格：200 Preps

价格：6320

产品类型：RNA提取

品牌：Zymo

TheDirect-zol^™RNA Kitsprovide a streamlined method for the purification of up to 100 µg (per prep) of high-quality RNAdirectlyfrom samples in TRI Reagent^®or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids,etc.).

Isolation of RNA by conventional phase separation was shownto selectively enrich for some species of miRNA, leading to bias in downstream analysis. TheDirect-zol^™method assures unbiased recovery of small RNAs including miRNA (see below).

The procedure is easy. Simply apply a prepared sample in TRI Reagent^®directly to theZymo-Spin^™Columnand then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencingetc.).

- Quick, spin column purification of high-quality (DNA-free) total RNA directly from TRIzol®, TRI Reagent® and other acid-guanidinium-phenol based reagents (RNAzol®, QIAzol®, TriPure™, TriSure™, etc.).

- Bypasses phase separation and precipitation procedures, for non-biased recovery of miRNA.

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数据资料

High quality broad range RNA is purified with the Direct-zol™ RNA MiniPrep.(A) DNA-free RNA purified from human epithelial cells using the Direct-zol™ RNA MiniPrep compared to a DNA containing preparation from Supplier Q (1% agarose/TAE). (B) Small RNAs are effectively recovered with the Direct-zol™ procedure while absent in Supplier Q preparations (Agilent Bioanalyzer 2100, Small RNA Chip data shown)

The data show RNA purified from TRIzol® samples using the Direct-zol™ RNA MiniPrep compared to an unbiased method (mirVana™, Ambion). Micro-RNA analysis was performed using miRNA-Seq (MiSeq®, Illumina) and a direct hybridization assay (nCounter®, Nanostring).