货号：109813

规格：25 µg

价格：420

产品类型：流式抗体

品牌：Biolegend

物种：小鼠

宿主：仓鼠

抗体亚型：IgG

荧光染料：APC

Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

• Isotype Control

• APC Mouse IgG2a, κ Isotype Ctrl (FC)

• Reactivity

• Mouse

• Immunogen

• B10.S mouse thymocytes and splenocytes

• Formulation

• Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

• Preparation

• The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.

• Concentration

• 0.2 mg/ml

• Storage & Handling

• The CD45.2 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.

• Application

• FC - Quality tested

• Recommended Usage

• Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

• Excitation Laser

• Red Laser (633 nm)

• Application Notes

• The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4,in vivoandin vitroblocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3.

• Application References(PubMed link indicates BioLegend citation)

• 1. Yakura H,et al.1983.J. Exp. Med.157:1077. (Block)2. Yakura H,et al.1986.J. Immunol.136:2729. (Block)3. Suzuki K,et al.2000.Immunity13:691. (IHC)4. Shen FW,et al.1986.Immunogenetics24:146. (IP)5. Baldwin TA and Hogquist KA. 2007.J. Immunol.179:837.6. Pascal V,et al.2007.J. Immunol.179:1751.7. Burman AC,et al.2007.Blood110:1064.8. Kincaid EZ,et al.2007.J. Immunol.179:3187.9. Phan TG,et al.2007.Nature Immunol.8:992.10. Nakano-Yokomizo T,et al.2011.J. Exp Med.208:1661.PubMed11. Wen T,et al.2013.PNAS.110:6067.PubMed12. Kohlmeier JE,et al.2008.Immunity.29:101. (FC)PubMed13. Markey KA,et al.2014.J Immunol.192:5426.PubMed14. Le Brocq ML,et al.2014.J Immunol.192:6120.PubMed15. Guo H,et al.2014.J Leukoc Biol.96:419.PubMed16. Schaefer K,et al.2014.PLoS One.9:114824.PubMed