货号：328133

规格：25 tests

价格：1680

产品类型：流式抗体

品牌：Biolegend

物种：人

宿主：仓鼠

抗体亚型：IgG

荧光染料：PE

Description

CD90 is a 25-35 kD GPI-anchored protein, also known as Thy-1. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34+cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34+CD90+cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.

• Isotype Control

• PE/Dazzle™ 594 Mouse IgG1, κ Isotype Ctrl

• Reactivity

• Human, African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Swine (Pig, Porcine)

• Immunogen

• HEL cells

• Formulation

• Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

• Preparation

• The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions. The solution is free of unconjugated PE/Dazzle™ 594 and unconjugated antibody.

• Concentration

• Lot-specific (please contacttechnical supportfor concentration and total µg amount, or use ourLookuptool if you have a lot number.)

• Storage & Handling

• The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.

• Application

• FC - Quality tested

• Recommended Usage

• Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

• Excitation Laser

• Blue Laser (488 nm)

• Application Notes

• Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.

• Application References(PubMed link indicates BioLegend citation)

• 1. Craig W,et al.1993.J. Exp. Med.177:1331. (IP)2. Gundlach CW 4th,et al.2011.Bioconjug. Chem.22:1706. (Pig Reactivity)3. Touboul C,et al.2013.J. Transl. Med.11:28. (IF)4. Bradley JE,et al.2013.Lab Invest.93:365. (Epitope)5. Donnenberg VS,et al.2010.Cytometry B. Clin. Cytom.5:287. (IHC)