货号：304150

规格：100 µg

价格：1610

产品类型：流式抗体

品牌：Biolegend

物种：人

宿主：仓鼠

抗体亚型：IgG

荧光染料：APC

Description

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

• Isotype Control

• APC Mouse IgG2b, κ Isotype Ctrl

• Reactivity

• Human,Cross-Reactivity:Chimpanzee

• Formulation

• µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

• Preparation

• The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.

• Concentration

• µg sizes: 0.2 mg/mltest sizes: lot-specific (please contacttechnical supportfor concentration and total µg amount)

• Storage & Handling

• The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.

• Application

• FC - Quality tested

• Recommended Usage

• Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis.For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume.Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more atwww.biolegend.com/testsizeregarding the test size change.

• Excitation Laser

• Red Laser (633 nm)

• Application Notes

• Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3and formalin-fixed paraffin-embedded tissue sections4, and immunofluorescence15,16.

• Application References(PubMed link indicates BioLegend citation)

• 1. Knapp W,et al.1989. Leucocyte Typing IV. Oxford University Press. New York.2. Yamada T,et al.2002.J. Biol. Chem.277:28830. (WB, Block)3. Weninger W,et al.2003J. Immunol.170:4638. (IHC)4. Imanguli MM,et al.2009.Blood. 113:3620 (IHC)5. Roque S,et al.2007. J. Immunol.178:8028. (FC)PubMed6. Smeltz RB. 2007.J. Immunol.178:4786. (FC)PubMed7. Palendira U,et al.2008.Blood(FC)PubMed8. Kuttruff S,et al.2009.Blood113:358. (FC)PubMed10. Thakral D,et al.2008.J. Immunol.180:7431. (FC)PubMed11. Alanio C,et al.2010.Blood115:3718. (FC)PubMed12. Iannello A,et al.2010.J. Immunol.184:114. (FC)PubMed13. Yoshino N,et al.2000.Exp. Anim. (Tokyo)49:97. (FC)14. Guereau-de-Arellan M,et al.2011.Brain. 134:3578.PubMed15. Canque B,et al.2000.Blood96:3748. (IF)16. Imanguli MM,et al.2009.Blood13:3620. (IF)