CD279 (PD-1) Monoclonal Antibody (J43), APC-eFluor 780
货号：47-9985-80,47-9985-82
规格：25 μg,100 μg
价格：1323,3257
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：Armenian hamster仓鼠
抗体亚型：IgG
荧光染料：Alexa Fluor 488
| 抗体类型 | 一抗 | 同型对照： | Armenian Hamster IgG Isotype Control (eBio299Arm), APC-eFluor 780 |
| 浓度： | 0.2 mg/mL | 用法： | 0.25 μg/test(Flow) |
| 产品详细信息Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.Applications Reported: This J43 antibody has been reported for use in flow cytometric analysis.Applications Tested: This J43 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of theimmune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE. |
| 数据 |
| CD279 (PD-1) Antibody (47-9985-80) in FlowStaining of unstimulated (left) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0031-82andProduct # 16-0281-82)-stimulated (right) C57Bl/6 splenocytes with Anti-Mouse/Rat CD278 (ICOS) APC (Product # 17-9949-82) and 0.125 µg of Anti-Mouse CD279 (PD-1) APC-eFluor® 780. Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis. |
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| 参考文献： |
| 1.The Journal of experimental medicineThe programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice.AuthorsAnsari MJ,Salama AD,Chitnis T,Smith RN,Yagita H,Akiba H,Yamazaki T,Azuma M,Iwai H,Khoury SJ,Auchincloss H,Sayegh MH2.OncoimmunologyThe stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas."Published figure using CD279 (PD-1) monoclonal antibody (Product # 47-9985-82) in Flow Cytometry"3.CellInitiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells."Published figure using CD279 (PD-1) monoclonal antibody (Product # 47-9985-82) in Flow Cytometry"4.OncoimmunologyGrowth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression."Published figure using CD279 (PD-1) monoclonal antibody (Product # 47-9985-82) in Flow Cytometry"5.Cancer researchOncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer Vaccination."Published figure using CD279 (PD-1) monoclonal antibody (Product # 47-9985-82) in Flow Cytometry" |
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