货号：D3025

规格： 200 Preps

价格：3344

产品类型：DNA提取

品牌：Zymo

TheQuick-DNA™ Miniprep Kitis a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, plasma, buffy coat, buccal cells, cells from culture, and many biological liquid samples.

For processing, simply add the specially formulatedGenomic Lysis Bufferto a sample, vortex, and transfer the mixture to the suppliedZymo-Spin™ Column. There is no need for organic denaturants or Proteinase K digestion because of the unique lysis buffer system. The product featuresZymo-Spin^™technology to yield high-quality, purified DNA in just minutes (see below). PCR inhibitors are effectively removed during the purification process. DNA purified using theQuick-DNA™ Miniprep Kitis suitable for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.

- Quick purification of high quality DNA from whole blood, plasma, serum, body fluids, buffy coat, lymphocytes, swabs or cultured cells in less than 15 minutes using innovative Zymo-Spin™ column technology.

- Compatible with commonly used anticoagulants (i.e., EDTA, heparin, citrate).

- Unique extraction technology excludes the use of Proteinase K and organic denaturants.

- Isolated DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.

DNA isolated from porcine whole blood using theQuick-DNA™ Miniprep Kit.Equivalent amounts (100 µl) of blood were processed without Proteinase K using theQuick-gDNA™ MiniPrep in half the time as compared to the kits from suppliers A and B. Equal volumes of eluted DNA were then analyzed (in duplicate) in a 0.8% (w/v) TAE/agarose/ethidium bromide gel. The size marker "M" is a 1 kb ladder (Zymo Research).