IL-17F Monoclonal Antibody (eBio18F10), eFluor 660

货号：50-7471-80,50-7471-82

规格：25μg,100μg

价格：0,3615

产品类型：流式抗体

品牌：eBioscience

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eBio18F10

荧光染料：eFluor 660

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), eFluor 660
浓度：	0.2 mg/mL	用法：	0.25 µg/test (Flow)

产品详细信息Description: The eBio18F10 antibody reacts with mouse IL-17F. IL-17F is a 37 kD homodimer of the IL-17 family and a signature Th17 marker. Of all the six IL-17 family members, IL-17F and IL-17A share the strongest homology (50% amino acid identity), and the two genes are located in the same chromosomal region. Recent studies have demonstrated coordinated regulation of IL-17A and IL-17F during Th17 differentiation. Expression of IL-17F and IL-17A has been detected in activated human peripheral blood lymphocytes, specifically by activated human CD4+ T cells. In addition to IL-17A, differentiated Th17 cells also produce IL-17F and IL-22 upon re-activation. Like IL-17A, IL-17F has been linked with inflammatory diseases. IL-17F and IL-17A expression has been observed in tissue samples from various autoimmune diseases, such as rheumatoid arthritis, multiple sclerosis, psoriasis, inflammatory bowel disease, and asthma. IL-17F treatment of airway epithelium, vein endothelial cells, and fibroblasts has been reported to induce expression of IL-6, IL-8, GRO-alpha, ENA-78, TGF-beta, MCP-1, G-CSF, GM-CSF, and ICAM-1.Like IL-17A, IL-17F is a disulfide-linked homodimeric glycoprotein. The IL-17F homodimer includes a classical cysteine knot motif, which is found also in the TGF-beta, BMP, and NGF superfamilies. The presence of the cysteine knot motif suggested the possibility of a heterodimeric structure, as was reported for TGF-beta and inhibin/activin. Recent reports confirm that co-expression of IL-17F and IL-17A in HEK293 cells results in the formation of biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F homodimers and IL-17A homodimers. Moreover, activated human CD4+ T cells were found to produce the IL-17A/F heterodimer, along with the corresponding homodimers. In comparing the relative potency of IL-17A, IL-17F, and IL-17A/F, all three were found to induce GRO-alpha secretion; IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100-fold lower than IL-17A). In the mouse, the IL-17A/F heterodimer (alone or in synergy with TNF-alpha) was found to regulate the expression of IL-6 and KC (mouse homolog of human GRO-alpha); this was found to be dependent on IL-17RA and TRAF6.Applications Reported: This eBio18F10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This eBio18F10 antibody has been tested by intracellular staining and flow cytometric analysis of Th17-polarized mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息IL-17F (Interleukin 17F, CTLA-8)) is a cytokine belonging to the IL-17 family that is produced by inflammatory cells such as activated T cells, mast cells, and basophils. IL-17F is involved in allergic airway inflammation, and can induce several cytokines, chemokines, and adhesion molecules in bronchial epithelial cells, vein endothelial cells, fibroblasts, and eosinophils. IL-17F may be secreted as a homodimer, or a heterodimer with IL17A. It acts by binding to the type I receptor, IL-17R, aiding recruitment of monocytes and neutrophils at the site of inflammation by increasing chemokine production. IL-17F also stimulates induction of other pro-inflammatory cytokines TNF alpha, IL-1 beta, IL-6, and IL-8, and reports strongly suggest the involvement of IL-17 in several chronic inflammatory diseases such as rheumatoid arthritis, psoriasis and multiple sclerosis. TGF-beta (differentiation) and IL-23 (expansion) are required for induction and maintenance of Th17 (IL-17 producing) cells, which in turn induce the other pro-inflammatory cytokines. IL-17F is produced, and exists, as a homo-dimer, with homology to a herpes virus early protein, is one of the six members (IL-17A-F) of this cytokine family, and is well characterized and highly expressed by activated effector memory T cells. IL-17F has been found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1.

数据

IL-17F Antibody (50-7471-80) in FlowIntracellular staining of Th17-polarized mouse splenocytes cultured with Brefeldin A (Product # 00-4506-51) alone (left) or stimulated for 5 hours with PMA/Ionomycin in the presence of Brefeldin A (right) followed by intracellular staining with Anti-Mouse CD4 PerCP-Cy5-5 (Product # 45-0042-82) and 0.125 µg of Anti-Mouse IL-17F eFluor® 660. Total viable cells as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14) were used for analysis.

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参考文献：

1.Mucosal immunologyRegulatory T cells promote a protective Th17-associated immune response to intestinal bacterial infection with C. rodentium."50-7471 was used in Flow cytometry/Cell sorting to study the impact of Foxp3(+) Tregs on inflammatory processes elicited by the bacterial pathogen C. rodentium, showing that Tregs promote a protective Th17-associated immune response."AuthorsWang Z,Friedrich C,Hagemann SC,Korte WH,Goharani N,Cording S,Eberl G,Sparwasser T,Lochner M2.NatureGeneration of pathogenic T(H)17 cells in the absence of TGF-β signalling. AuthorsGhoreschi K,Laurence A,Yang XP,Tato CM,McGeachy MJ,Konkel JE,Ramos HL,Wei L,Davidson TS,Bouladoux N,Grainger JR,Chen Q,Kanno Y,Watford WT,Sun HW,Eberl G,Shevach EM,Belkaid Y,Cua DJ,Chen W,O'Shea JJ4.ImmunityLate developmental plasticity in the T helper 17 lineage.

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