anti-mouse CD19 (eBio1D3) eFluor 660
货号：50-0193-80,50-0193-82
规格：25 μg,100 μg
价格：0,2019
产品类型：流式抗体
品牌：eBioscience
抗原：CD19
物种：小鼠
宿主：大鼠
抗体亚型：IgG
克隆号：eBio1D3 (1D3)
荧光染料：eFluor 660
| 抗体类型: | 单抗 | 同型对照： | IgG2a, kappa |
| 免疫原性： | | 应用领域： | Flow；MISC；FN |
| 推荐用法： | 0.25 µg/test（flow) |
| Description: The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD19 Antibody (50-0193-82) Staining of BALB/c splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.125 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.125 µg of Anti-Mouse CD19 eFluor® 660 (right). Total viable cells were used for analysis. |
| 推荐产品： |
| Flow Cytometry Staining Buffer, eBioscience Ultracomp eBeads microspheres LIVE/DEAD® Fixable Cell Stains |
| 参考文献： |
| 1.The EMBO journal Molecular requirements of the B-cell antigen receptor for sensing monovalent antigens."50-0193 was used in Flow cytometry/Cell sorting to study the effects of differing monovalent stimuli on B-cell antigen receptor signalling and organisation."Authors Volkmann C,Brings N,Becker M,Hobeika E,Yang J,Reth M2. Journal of immunology (Baltimore, Md. : 1950) The signaling activity of murine CD19 is regulated during cell development.Authors Krop I,Shaffer AL,Fearon DT,Schlissel MS3.European journal of immunology Self-renewal of B-1 lymphocytes is dependent on CD19.Authors Krop I,de Fougerolles AR,Hardy RR,Allison M,Schlissel MS,Fearon DT |
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