anti-mouse CD19 (EBIO1D3) PE-eFluor610
货号：61-0193-80,61-0193-82
规格：25ug,100ug
价格：0,2848
产品类型：流式抗体
品牌：eBioscience
抗原：CD19
物种：小鼠
宿主：小鼠
抗体亚型：IgG
克隆号： eBio1D3 (1D3)
荧光染料：PE-eFluor 610
| 抗体类型: | 单抗 | 同型对照： | IgG2a, kappa |
| 免疫原性： | | 应用领域： | Flow；MISC；FN |
| 推荐用法： | 0.25 µg/test（flow) |
| Description: The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD19 Antibody (61-0193-82) Staining of mouse splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.125 µg of Anti-Mouse CD19 PE-eFluor® 610. Cells in the lymphocyte gate were used for analysis. |
| 推荐产品： |
| Flow Cytometry Staining Buffer, eBioscienceUltracomp eBeads microspheresLIVE/DEAD® Fixable Cell Stains |
| 参考文献： |
| 1. PloS one Viral Infection of the Central Nervous System Exacerbates Interleukin-10 Receptor Deficiency-Mediated Colitis in SJL Mice. "61019382 was used in flow cytometry to study the effect of IL-10 signaling in infectious neurological diseases" Authors Uhde AK,Herder V,Akram Khan M,Ciurkiewicz M,Schaudien D,Teich R,Floess S,Baumgärtner W,Huehn J,Beineke A2. Journal of immunology (Baltimore, Md. : 1950) The signaling activity of murine CD19 is regulated during cell development. Authors Krop I,Shaffer AL,Fearon DT,Schlissel MS3. European journal of immunology Self-renewal of B-1 lymphocytes is dependent on CD19. Authors Krop I,de Fougerolles AR,Hardy RR,Allison M,Schlissel MS,Fearon DT4. Journal of immunology (Baltimore, Md. : 1950) The signaling activity of murine CD19 is regulated during cell development. Authors Krop I,Shaffer AL,Fearon DT,Schlissel MS |
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