Mouse anti-Human IgA (Heavy chain) Secondary Antibody/鼠抗人IgA（重链）二抗
货号：MA5-11208
规格：500 µL
价格：4023
产品类型：二抗
品牌：Thermo Fisher
抗原： Purified human alpha heavy chain
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：
荧光染料：其它
| 抗体类型: | 二抗 | 同型对照： | |
| 浓度： | 0.2 mg/mL | 应用领域： | 酶联免疫吸附实验
(ELISA) 1 µg/mL 流式细胞分析
(Flow) 2 µg/test 免疫组化（石蜡）
(IHC (P)) 1:12.5-1:25 免疫印迹
(WB) 1:00-1:1000 |
| By Western blot, MA5-11208 detects Human IgA (alpha) heavy chain at ~55kD in human, but not mouse samples.By ELISA and WB, MA5-11208 does not react with other human Ig isotypes.靶标信息Thermo Scientific Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. |
| 数据： |
| Human IgA (Heavy chain) Secondary Antibody (MA5-11208) in IHC Formalin-fixed, paraffin-embedded human tonsil stained with IgA antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of IgA secreting cells.Human IgA (Heavy chain) Secondary Antibody (MA5-11208) in WB Western blot analysis of IgA was performed by loading 0.5 µg of purified Human IgA, Human IgG, Human IgM, and Mouse IgA, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to PVDF membranes using the G2 Fast Blotter (Product # 62288), and blocked with 5% BSA in TBST for at least 1 hour at room temperature. IgA heavy chain was detected at ~55 kD using a mouse anti-human IgA monoclonal antibody (Product # MA5-11208) diluted to a concentration of 0.5 µg/mL in blocking buffer, overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse light chain secondary antibody diluted 1:40,000 for at least 30 minutes at room temperature (panel A). To verify the presence of all isotypes, a second blot was probed with a goat anti-human IgM + IgG +IgA polyclonal antibody (Product # 31128) at a concentration of 0.5 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated mouse anti-goat IgG secondary antibody (Product # 31400) diluted 1:40,000 in blocking buffer for at least 30 minutes at room temperature (panel B).Chemiluminescent detection was performed using SuperSignal West Pico.Human IgA (Heavy chain) Secondary Antibody (MA5-11208) in WB Western blot analysis of IgA was performed by loading 25 µg of various human tissue lysates, 0.5 µg of Human IgA, 0.25 µL of human serum, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% BSA in TBST for at least 1 hour at room temperature. IgA heavy chain was detected at ~55 kD using a mouse anti-human IgA monoclonal antibody (Product # MA5-11208) diluted to a concentration of 0.5 µg/mL in blocking buffer, overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse light chain secondary antibody diluted 1:40,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico. *The band at ~25 kD is a result of spill over from an adjacent lane.Human IgA (Heavy chain) Secondary Antibody (MA5-11208) in WB Western blot analysis of Human IgA (Alpha heavy chain) was performed by loading 25 µg of Daudi cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Mouse anti-Human IgA (alpha-Heavy Chain) Secondary antibody (Product # MA5-11208) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~60 kDa.Human IgA (Heavy chain) Secondary Antibody (MA5-11208) in Flow Flow cytometry analysis of Human IgA (alpha-Heavy Chain) in Daudi cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Mouse anti-Human IgA (alpha-Heavy Chain) Secondary antibody (Product # MA5-11208) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis. |
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| 参考文献： |
| 1.Proceedings of the National Academy of Sciences of the United States of AmericaGrowth hormone is permissive for neoplastic colon growth. |
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